Identification of focal viral infections by confocal microscopy for subsequent ultrastructural analysis.

Journal Article

A correlative microscopy method for the ultrastructural analysis of focal viral tissue infections is presented. Using a confocal scanning laser microscope, foci of infection are identified in tissue sections prior to embedment; a variety of techniques can be employed for viral detection, including staining with standard histochemical reagents and fluorescently labeled antibodies. Areas of infection identified using confocal microscopy are excised from the tissue sections, embedded, and examined by transmission electron microscopy. Applications of this technique in both diagnostic and basic research settings are described.

Full Text

Duke Authors

Cited Authors

  • Miller, SE; Levenson, RM; Aldridge, C; Hester, S; Kenan, DJ; Howell, DN

Published Date

  • March 1997

Published In

Volume / Issue

  • 21 / 2

Start / End Page

  • 183 - 193

PubMed ID

  • 9090029

International Standard Serial Number (ISSN)

  • 0191-3123

Language

  • eng

Conference Location

  • England