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Bacteria-host cell interaction mediated by cellular cholesterol/glycolipid-enriched microdomains.

Publication ,  Journal Article
Shin, JS; Gao, Z; Abraham, SN
Published in: Biosci Rep
October 1999

Gram negative bacterial infection is a leading cause of fatality and is attributed, at least in part, to the bacteria's capacity to persist in the host in spite of appropriate antibiotic therapy. It has been suggested that bacteria evade antibiotics by hiding within host cells. We sought to investigate this important aspect of infections in mast cells, which are inflammatory cells found in close proximity to the host-environment interface and which have recently been reported to play a crucial role in the early innate immune response to bacteria. We examined mast cell interactions with FimH-expressing E. coli, one of the major opportunistic pathogens of humans. We determined that in serum free conditions, these bacteria were able to trigger mast cell uptake without loss of bacterial viability. CD48, a mannose containing GPI (glycosylphosphatidylinositol)-linked molecule was found to be the receptor of FimH-expressing E. coli in mouse mast cells. We found that the internalization via CD48 was blocked by filipin, a cholesterol binding drug known to disrupt cholesterol/glycolipid-enriched microdomains and the bacteria-encasing vacuoles were rich in cholesterol inside cells. Interestingly, we found that mast cells subsequently expelled majority of the intracellular bacteria in 24 hours. This expulsion process was blocked by lovastatin/cyclodextrin treatment, which is known to inhibit cellular trafficking of cholesterol/glycolipid-enriched microdomains. Thus, the bacterial entry into and expulsion from mast cells were critically dependent on cholesterol/glycolipid-enriched microdomains, which represents a novel mode of tussle between the pathogen and the mast cell occurring in opsonin deficient sites in the body or even at other sites in naive or immunocompromised hosts which have low systemic levels of E. coli specific antibody.

Duke Scholars

Published In

Biosci Rep

DOI

ISSN

0144-8463

Publication Date

October 1999

Volume

19

Issue

5

Start / End Page

421 / 432

Location

England

Related Subject Headings

  • Phagocytosis
  • Opsonin Proteins
  • Mice
  • Mast Cells
  • Humans
  • Glycolipids
  • Fimbriae Proteins
  • Escherichia coli
  • Cholesterol
  • Cells, Cultured
 

Citation

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ICMJE
MLA
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Shin, J. S., Gao, Z., & Abraham, S. N. (1999). Bacteria-host cell interaction mediated by cellular cholesterol/glycolipid-enriched microdomains. Biosci Rep, 19(5), 421–432. https://doi.org/10.1023/a:1020216323271
Shin, J. S., Z. Gao, and S. N. Abraham. “Bacteria-host cell interaction mediated by cellular cholesterol/glycolipid-enriched microdomains.Biosci Rep 19, no. 5 (October 1999): 421–32. https://doi.org/10.1023/a:1020216323271.
Shin JS, Gao Z, Abraham SN. Bacteria-host cell interaction mediated by cellular cholesterol/glycolipid-enriched microdomains. Biosci Rep. 1999 Oct;19(5):421–32.
Shin, J. S., et al. “Bacteria-host cell interaction mediated by cellular cholesterol/glycolipid-enriched microdomains.Biosci Rep, vol. 19, no. 5, Oct. 1999, pp. 421–32. Pubmed, doi:10.1023/a:1020216323271.
Shin JS, Gao Z, Abraham SN. Bacteria-host cell interaction mediated by cellular cholesterol/glycolipid-enriched microdomains. Biosci Rep. 1999 Oct;19(5):421–432.
Journal cover image

Published In

Biosci Rep

DOI

ISSN

0144-8463

Publication Date

October 1999

Volume

19

Issue

5

Start / End Page

421 / 432

Location

England

Related Subject Headings

  • Phagocytosis
  • Opsonin Proteins
  • Mice
  • Mast Cells
  • Humans
  • Glycolipids
  • Fimbriae Proteins
  • Escherichia coli
  • Cholesterol
  • Cells, Cultured