Glycerol-induced unraveling of the tight helical conformation of Escherichia coli type 1 fimbriae.

Published

Journal Article

Glycerol was found to unravel the helical conformation of Escherichia coli type 1 fimbriae without appreciable depolymerization. The linearized fimbrial polymers have a diameter of 2 nm, react strongly with a monoclonal antibody directed at an inaccessible epitope on native fimbriae, and display greater mannose-binding activity and trypsin sensitivity than native fimbriae. Removal of glycerol by dialysis results in spontaneous reassembly of the linear polymers into structures morphologically, antigenically, and functionally indistinguishable from native fimbriae.

Full Text

Duke Authors

Cited Authors

  • Abraham, SN; Land, M; Ponniah, S; Endres, R; Hasty, DL; Babu, JP

Published Date

  • August 1992

Published In

Volume / Issue

  • 174 / 15

Start / End Page

  • 5145 - 5148

PubMed ID

  • 1352770

Pubmed Central ID

  • 1352770

International Standard Serial Number (ISSN)

  • 0021-9193

Digital Object Identifier (DOI)

  • 10.1128/jb.174.15.5145-5148.1992

Language

  • eng

Conference Location

  • United States