Involvement of cellular caveolae in bacterial entry into mast cells.

Published

Journal Article

Caveolae are subcellular structures implicated in the import and transcytosis of macromolecules and in transmembrane signaling. To date, evidence for the existence of caveolae in hematopoietic cells has been ambiguous. Caveolae were detected in the microvilli and intracellular vesicles of cultured mouse bone marrow-derived mast cells (BMMCs). CD48, a receptor for FimH-expressing (type 1 fimbriated) Escherichia coli, was specifically localized to plasmalemmal caveolae in BMMCs. The involvement of caveolae in bacterial entry into BMMCs was indicated because caveolae-disrupting and -usurping agents specifically blocked E. coli entry, and markers of caveolae were actively recruited to sites of bacterial entry. The formation of bacteria-encapsulating caveolar chambers in BMMCs represents a distinct mechanism of microbial entry into phagocytes.

Full Text

Duke Authors

Cited Authors

  • Shin, JS; Gao, Z; Abraham, SN

Published Date

  • August 4, 2000

Published In

Volume / Issue

  • 289 / 5480

Start / End Page

  • 785 - 788

PubMed ID

  • 10926542

Pubmed Central ID

  • 10926542

International Standard Serial Number (ISSN)

  • 0036-8075

Digital Object Identifier (DOI)

  • 10.1126/science.289.5480.785

Language

  • eng

Conference Location

  • United States