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Role of mast cell leukotrienes in neutrophil recruitment and bacterial clearance in infectious peritonitis.

Publication ,  Journal Article
Malaviya, R; Abraham, SN
Published in: J Leukoc Biol
June 2000

Stimulated mast cells release a variety of chemotactic factors such as tumor necrosis factor alpha (TNF-alpha) and leukotriene B4. Recent studies have shown that mast cell-derived TNF-alpha plays a critical role in host defense against Gram negative bacterial infections by the recruitment of neutrophils to the sites of infection. In the present study, we sought to investigate if mast cells release leukotriene (LT) B4 in response to bacteria and, if so, to establish its in vivo relevance. We show that mast cells release significant amounts of LTB4 and LTC4 in response to exposure to FimH-expressing type 1 fimbriated Escherichia coli in vitro. To test the functional significance of mast cell-derived LTs during an E. coli infection in vivo, we examined the effect of a LT-synthesis inhibitor, A-63162, on bacterial clearance and neutrophil influx in an infectious peritonitis model in mast cell-deficient mice (WBB6F1-W/WV) and their normal congenic control (WBB6F1-+/+) mice. Our results show that a treatment with A-63162 reduced neutrophil influx and bacterial clearance in the peritoneal cavities of mast cell-sufficient but not -deficient mice. Thus, mast cell-derived LTs contribute to host defense by mediating early neutrophil influx and bacterial clearance at sites of infection.

Duke Scholars

Published In

J Leukoc Biol

DOI

ISSN

0741-5400

Publication Date

June 2000

Volume

67

Issue

6

Start / End Page

841 / 846

Location

England

Related Subject Headings

  • Peritonitis
  • Neutrophils
  • Mice, Inbred C57BL
  • Mice, Inbred BALB C
  • Mice
  • Mast Cells
  • Male
  • Leukotriene C4
  • Leukotriene B4
  • Immunology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Malaviya, R., & Abraham, S. N. (2000). Role of mast cell leukotrienes in neutrophil recruitment and bacterial clearance in infectious peritonitis. J Leukoc Biol, 67(6), 841–846. https://doi.org/10.1002/jlb.67.6.841
Malaviya, R., and S. N. Abraham. “Role of mast cell leukotrienes in neutrophil recruitment and bacterial clearance in infectious peritonitis.J Leukoc Biol 67, no. 6 (June 2000): 841–46. https://doi.org/10.1002/jlb.67.6.841.
Malaviya, R., and S. N. Abraham. “Role of mast cell leukotrienes in neutrophil recruitment and bacterial clearance in infectious peritonitis.J Leukoc Biol, vol. 67, no. 6, June 2000, pp. 841–46. Pubmed, doi:10.1002/jlb.67.6.841.

Published In

J Leukoc Biol

DOI

ISSN

0741-5400

Publication Date

June 2000

Volume

67

Issue

6

Start / End Page

841 / 846

Location

England

Related Subject Headings

  • Peritonitis
  • Neutrophils
  • Mice, Inbred C57BL
  • Mice, Inbred BALB C
  • Mice
  • Mast Cells
  • Male
  • Leukotriene C4
  • Leukotriene B4
  • Immunology