Assembly of a chemically synthesized peptide of Escherichia coli type 1 fimbriae into fimbria-like antigenic structures.
Publication
, Journal Article
Abraham, SN; Beachey, EH
Published in: J Bacteriol
June 1987
Escherichia coli type 1 fimbriae are composed of subunits, each of which comprises 158 amino acids. We synthesized a copy of a 13-residue peptide, located near the NH2 terminus of the fimbrial subunit, that assumed some of the properties of type 1 fimbriae. At pH 5.5 the synthetic peptide autoassembled into fibrillar structures that resembled type 1 fimbriae except that they appeared less rigid and rodlike. A quaternary structure-specific monoclonal antibody against type 1 fimbriae recognized the synthetic peptide in the assembled but not the unassembled state. Furthermore, when the synthetic peptide was injected in its fimbrial conformation into rabbits, it evoked antibodies that reacted with type 1 fimbriae isolated from E. coli.
Duke Scholars
Published In
J Bacteriol
DOI
ISSN
0021-9193
Publication Date
June 1987
Volume
169
Issue
6
Start / End Page
2460 / 2465
Location
United States
Related Subject Headings
- Oligopeptides
- Molecular Weight
- Microscopy, Electron
- Microbiology
- Macromolecular Substances
- Haemophilus influenzae
- Fimbriae, Bacterial
- Escherichia coli
- Cross Reactions
- Bacterial Proteins
Citation
APA
Chicago
ICMJE
MLA
NLM
Abraham, S. N., & Beachey, E. H. (1987). Assembly of a chemically synthesized peptide of Escherichia coli type 1 fimbriae into fimbria-like antigenic structures. J Bacteriol, 169(6), 2460–2465. https://doi.org/10.1128/jb.169.6.2460-2465.1987
Abraham, S. N., and E. H. Beachey. “Assembly of a chemically synthesized peptide of Escherichia coli type 1 fimbriae into fimbria-like antigenic structures.” J Bacteriol 169, no. 6 (June 1987): 2460–65. https://doi.org/10.1128/jb.169.6.2460-2465.1987.
Abraham SN, Beachey EH. Assembly of a chemically synthesized peptide of Escherichia coli type 1 fimbriae into fimbria-like antigenic structures. J Bacteriol. 1987 Jun;169(6):2460–5.
Abraham, S. N., and E. H. Beachey. “Assembly of a chemically synthesized peptide of Escherichia coli type 1 fimbriae into fimbria-like antigenic structures.” J Bacteriol, vol. 169, no. 6, June 1987, pp. 2460–65. Pubmed, doi:10.1128/jb.169.6.2460-2465.1987.
Abraham SN, Beachey EH. Assembly of a chemically synthesized peptide of Escherichia coli type 1 fimbriae into fimbria-like antigenic structures. J Bacteriol. 1987 Jun;169(6):2460–2465.
Published In
J Bacteriol
DOI
ISSN
0021-9193
Publication Date
June 1987
Volume
169
Issue
6
Start / End Page
2460 / 2465
Location
United States
Related Subject Headings
- Oligopeptides
- Molecular Weight
- Microscopy, Electron
- Microbiology
- Macromolecular Substances
- Haemophilus influenzae
- Fimbriae, Bacterial
- Escherichia coli
- Cross Reactions
- Bacterial Proteins