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Assembly of a chemically synthesized peptide of Escherichia coli type 1 fimbriae into fimbria-like antigenic structures.

Publication ,  Journal Article
Abraham, SN; Beachey, EH
Published in: J Bacteriol
June 1987

Escherichia coli type 1 fimbriae are composed of subunits, each of which comprises 158 amino acids. We synthesized a copy of a 13-residue peptide, located near the NH2 terminus of the fimbrial subunit, that assumed some of the properties of type 1 fimbriae. At pH 5.5 the synthetic peptide autoassembled into fibrillar structures that resembled type 1 fimbriae except that they appeared less rigid and rodlike. A quaternary structure-specific monoclonal antibody against type 1 fimbriae recognized the synthetic peptide in the assembled but not the unassembled state. Furthermore, when the synthetic peptide was injected in its fimbrial conformation into rabbits, it evoked antibodies that reacted with type 1 fimbriae isolated from E. coli.

Duke Scholars

Published In

J Bacteriol

DOI

ISSN

0021-9193

Publication Date

June 1987

Volume

169

Issue

6

Start / End Page

2460 / 2465

Location

United States

Related Subject Headings

  • Oligopeptides
  • Molecular Weight
  • Microscopy, Electron
  • Microbiology
  • Macromolecular Substances
  • Haemophilus influenzae
  • Fimbriae, Bacterial
  • Escherichia coli
  • Cross Reactions
  • Bacterial Proteins
 

Citation

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Abraham, S. N., & Beachey, E. H. (1987). Assembly of a chemically synthesized peptide of Escherichia coli type 1 fimbriae into fimbria-like antigenic structures. J Bacteriol, 169(6), 2460–2465. https://doi.org/10.1128/jb.169.6.2460-2465.1987
Abraham, S. N., and E. H. Beachey. “Assembly of a chemically synthesized peptide of Escherichia coli type 1 fimbriae into fimbria-like antigenic structures.J Bacteriol 169, no. 6 (June 1987): 2460–65. https://doi.org/10.1128/jb.169.6.2460-2465.1987.
Abraham, S. N., and E. H. Beachey. “Assembly of a chemically synthesized peptide of Escherichia coli type 1 fimbriae into fimbria-like antigenic structures.J Bacteriol, vol. 169, no. 6, June 1987, pp. 2460–65. Pubmed, doi:10.1128/jb.169.6.2460-2465.1987.

Published In

J Bacteriol

DOI

ISSN

0021-9193

Publication Date

June 1987

Volume

169

Issue

6

Start / End Page

2460 / 2465

Location

United States

Related Subject Headings

  • Oligopeptides
  • Molecular Weight
  • Microscopy, Electron
  • Microbiology
  • Macromolecular Substances
  • Haemophilus influenzae
  • Fimbriae, Bacterial
  • Escherichia coli
  • Cross Reactions
  • Bacterial Proteins