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Drosophila APC2 is a cytoskeletally-associated protein that regulates wingless signaling in the embryonic epidermis.

Publication ,  Journal Article
McCartney, BM; Dierick, HA; Kirkpatrick, C; Moline, MM; Baas, A; Peifer, M; Bejsovec, A
Published in: The Journal of cell biology
September 1999

The tumor suppressor adenomatous polyposis coli (APC) negatively regulates Wingless (Wg)/Wnt signal transduction by helping target the Wnt effector beta-catenin or its Drosophila homologue Armadillo (Arm) for destruction. In cultured mammalian cells, APC localizes to the cell cortex near the ends of microtubules. Drosophila APC (dAPC) negatively regulates Arm signaling, but only in a limited set of tissues. We describe a second fly APC, dAPC2, which binds Arm and is expressed in a broad spectrum of tissues. dAPC2's subcellular localization revealed colocalization with actin in many but not all cellular contexts, and also suggested a possible interaction with astral microtubules. For example, dAPC2 has a striking asymmetric distribution in neuroblasts, and dAPC2 colocalizes with assembling actin filaments at the base of developing larval denticles. We identified a dAPC2 mutation, revealing that dAPC2 is a negative regulator of Wg signaling in the embryonic epidermis. This allele acts genetically downstream of wg, and upstream of arm, dTCF, and, surprisingly, dishevelled. We discuss the implications of our results for Wg signaling, and suggest a role for dAPC2 as a mediator of Wg effects on the cytoskeleton. We also speculate on more general roles that APCs may play in cytoskeletal dynamics.

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Published In

The Journal of cell biology

DOI

EISSN

1540-8140

ISSN

0021-9525

Publication Date

September 1999

Volume

146

Issue

6

Start / End Page

1303 / 1318

Related Subject Headings

  • Wnt1 Protein
  • Tumor Cells, Cultured
  • Transcription Factors
  • Trans-Activators
  • Spindle Apparatus
  • Signal Transduction
  • Proto-Oncogene Proteins
  • Phosphorylation
  • Neurons
  • Mutation
 

Citation

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McCartney, B. M., Dierick, H. A., Kirkpatrick, C., Moline, M. M., Baas, A., Peifer, M., & Bejsovec, A. (1999). Drosophila APC2 is a cytoskeletally-associated protein that regulates wingless signaling in the embryonic epidermis. The Journal of Cell Biology, 146(6), 1303–1318. https://doi.org/10.1083/jcb.146.6.1303
McCartney, B. M., H. A. Dierick, C. Kirkpatrick, M. M. Moline, A. Baas, M. Peifer, and A. Bejsovec. “Drosophila APC2 is a cytoskeletally-associated protein that regulates wingless signaling in the embryonic epidermis.The Journal of Cell Biology 146, no. 6 (September 1999): 1303–18. https://doi.org/10.1083/jcb.146.6.1303.
McCartney BM, Dierick HA, Kirkpatrick C, Moline MM, Baas A, Peifer M, et al. Drosophila APC2 is a cytoskeletally-associated protein that regulates wingless signaling in the embryonic epidermis. The Journal of cell biology. 1999 Sep;146(6):1303–18.
McCartney, B. M., et al. “Drosophila APC2 is a cytoskeletally-associated protein that regulates wingless signaling in the embryonic epidermis.The Journal of Cell Biology, vol. 146, no. 6, Sept. 1999, pp. 1303–18. Epmc, doi:10.1083/jcb.146.6.1303.
McCartney BM, Dierick HA, Kirkpatrick C, Moline MM, Baas A, Peifer M, Bejsovec A. Drosophila APC2 is a cytoskeletally-associated protein that regulates wingless signaling in the embryonic epidermis. The Journal of cell biology. 1999 Sep;146(6):1303–1318.

Published In

The Journal of cell biology

DOI

EISSN

1540-8140

ISSN

0021-9525

Publication Date

September 1999

Volume

146

Issue

6

Start / End Page

1303 / 1318

Related Subject Headings

  • Wnt1 Protein
  • Tumor Cells, Cultured
  • Transcription Factors
  • Trans-Activators
  • Spindle Apparatus
  • Signal Transduction
  • Proto-Oncogene Proteins
  • Phosphorylation
  • Neurons
  • Mutation