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Overexpression of AtCPS and AtKS in Arabidopsis confers increased ent-kaurene production but no increase in bioactive gibberellins.

Publication ,  Journal Article
Fleet, CM; Yamaguchi, S; Hanada, A; Kawaide, H; David, CJ; Kamiya, Y; Sun, T-P
Published in: Plant physiology
June 2003

The plant growth hormone gibberellin (GA) is important for many aspects of plant growth and development. Although most genes encoding enzymes at each step of the GA biosynthetic pathway have been cloned, their regulation is less well understood. To assess how up-regulation of early steps affects the biosynthetic pathway overall, we have examined transgenic Arabidopsis plants that overexpress either AtCPS or AtKS or both. These genes encode the enzymes ent-copalyl diphosphate synthase (CPS) and ent-kaurene synthase, which catalyze the first two committed steps in GA biosynthesis. We find that both CPS and CPS/ent-kaurene synthase overexpressors have greatly increased levels of the early intermediates ent-kaurene and ent-kaurenoic acid, but a lesser increase of later metabolites. These overexpression lines do not exhibit any GA overdose morphology and have wild-type levels of bioactive GAs. Our data show that CPS is limiting for ent-kaurene production and suggest that conversion of ent-kaurenoic acid to GA12 by ent-kaurenoic acid oxidase may be an important rate-limiting step for production of bioactive GA. These results demonstrate the ability of plants to maintain GA homeostasis despite large changes in accumulation of early intermediates in the biosynthetic pathway.

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Published In

Plant physiology

DOI

EISSN

1532-2548

ISSN

0032-0889

Publication Date

June 2003

Volume

132

Issue

2

Start / End Page

830 / 839

Related Subject Headings

  • Polymerase Chain Reaction
  • Plant Proteins
  • Plant Biology & Botany
  • Gibberellins
  • Genes, Synthetic
  • Genes, Plant
  • Gene Expression Regulation, Plant
  • Gene Expression Regulation, Enzymologic
  • Arabidopsis
  • Alkyl and Aryl Transferases
 

Citation

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Fleet, C. M., Yamaguchi, S., Hanada, A., Kawaide, H., David, C. J., Kamiya, Y., & Sun, T.-P. (2003). Overexpression of AtCPS and AtKS in Arabidopsis confers increased ent-kaurene production but no increase in bioactive gibberellins. Plant Physiology, 132(2), 830–839. https://doi.org/10.1104/pp.103.021725
Fleet, Christine M., Shinjiro Yamaguchi, Atsushi Hanada, Hiroshi Kawaide, Charles J. David, Yuji Kamiya, and Tai-Ping Sun. “Overexpression of AtCPS and AtKS in Arabidopsis confers increased ent-kaurene production but no increase in bioactive gibberellins.Plant Physiology 132, no. 2 (June 2003): 830–39. https://doi.org/10.1104/pp.103.021725.
Fleet CM, Yamaguchi S, Hanada A, Kawaide H, David CJ, Kamiya Y, et al. Overexpression of AtCPS and AtKS in Arabidopsis confers increased ent-kaurene production but no increase in bioactive gibberellins. Plant physiology. 2003 Jun;132(2):830–9.
Fleet, Christine M., et al. “Overexpression of AtCPS and AtKS in Arabidopsis confers increased ent-kaurene production but no increase in bioactive gibberellins.Plant Physiology, vol. 132, no. 2, June 2003, pp. 830–39. Epmc, doi:10.1104/pp.103.021725.
Fleet CM, Yamaguchi S, Hanada A, Kawaide H, David CJ, Kamiya Y, Sun T-P. Overexpression of AtCPS and AtKS in Arabidopsis confers increased ent-kaurene production but no increase in bioactive gibberellins. Plant physiology. 2003 Jun;132(2):830–839.

Published In

Plant physiology

DOI

EISSN

1532-2548

ISSN

0032-0889

Publication Date

June 2003

Volume

132

Issue

2

Start / End Page

830 / 839

Related Subject Headings

  • Polymerase Chain Reaction
  • Plant Proteins
  • Plant Biology & Botany
  • Gibberellins
  • Genes, Synthetic
  • Genes, Plant
  • Gene Expression Regulation, Plant
  • Gene Expression Regulation, Enzymologic
  • Arabidopsis
  • Alkyl and Aryl Transferases