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Synergistic derepression of gibberellin signaling by removing RGA and GAI function in Arabidopsis thaliana.

Publication ,  Journal Article
Dill, A; Sun, T
Published in: Genetics
October 2001

RGA and GAI are negative regulators of the gibberellin (GA) signal transduction pathway in Arabidopsis thaliana. These genes may have partially redundant functions because they are highly homologous, and plants containing single null mutations at these loci are phenotypically similar to wild type. Previously, rga loss-of-function mutations were shown to partially suppress defects of the GA-deficient ga1-3 mutant. Phenotypes rescued include abaxial trichome initiation, rosette radius, flowering time, stem elongation, and apical dominance. Here we present work showing that the rga-24 and gai-t6 null mutations have a synergistic effect on plant growth. Although gai-t6 alone has little effect, when combined with rga-24, they completely rescued the above defects of ga1-3 to wild-type or GA-overdose phenotype. However, seed germination and flower development defects were not restored. Additionally, rga-24 and rga-24/gai-t6 but not gai-t6 alone caused increased feedback inhibition of expression of a GA biosynthetic gene in both the ga1-3 and wild-type backgrounds. These results demonstrate that RGA and GAI have partially redundant functions in maintaining the repressive state of the GA-signaling pathway, but RGA plays a more dominant role than GAI. Removing both RGA and GAI function allows for complete derepression of many aspects of GA signaling.

Duke Scholars

Published In

Genetics

DOI

EISSN

1943-2631

ISSN

0016-6731

Publication Date

October 2001

Volume

159

Issue

2

Start / End Page

777 / 785

Related Subject Headings

  • Signal Transduction
  • Repressor Proteins
  • Plant Proteins
  • Phenotype
  • Gibberellins
  • Germination
  • Drosophila Proteins
  • Developmental Biology
  • DNA Primers
  • Cell Cycle Proteins
 

Citation

APA
Chicago
ICMJE
MLA
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Dill, A., & Sun, T. (2001). Synergistic derepression of gibberellin signaling by removing RGA and GAI function in Arabidopsis thaliana. Genetics, 159(2), 777–785. https://doi.org/10.1093/genetics/159.2.777
Dill, A., and T. Sun. “Synergistic derepression of gibberellin signaling by removing RGA and GAI function in Arabidopsis thaliana.Genetics 159, no. 2 (October 2001): 777–85. https://doi.org/10.1093/genetics/159.2.777.
Dill, A., and T. Sun. “Synergistic derepression of gibberellin signaling by removing RGA and GAI function in Arabidopsis thaliana.Genetics, vol. 159, no. 2, Oct. 2001, pp. 777–85. Epmc, doi:10.1093/genetics/159.2.777.

Published In

Genetics

DOI

EISSN

1943-2631

ISSN

0016-6731

Publication Date

October 2001

Volume

159

Issue

2

Start / End Page

777 / 785

Related Subject Headings

  • Signal Transduction
  • Repressor Proteins
  • Plant Proteins
  • Phenotype
  • Gibberellins
  • Germination
  • Drosophila Proteins
  • Developmental Biology
  • DNA Primers
  • Cell Cycle Proteins