Snare protein expression and adenoviral transfection of amphicrine AR42J.

Journal Article

The amphicrine AR42J acinar cell line is an excellent model to study both exocrine and neuroendocrine exocytotic mechanisms. As a first step toward this goal, we determined the specific isoforms of the v- and t-SNARE and Munc18 families expressed in these cells. In addition, we show that dexamethasone-induced differentiation toward the exocrine phenotype causes an upregulation of several of these proteins. AR42J is notoriously difficult to transfect, limiting its usefulness as a model. However, we have now overcome this obstacle by acheiving high efficiency expression of a beta-galactosidase reporter gene and truncated SNAP-25 gene using adenoviral infection techniques. The AR42J cells can now be used to pursue and elucidate the distinct functions of individual SNARE isoforms used in endocrine and exocrine secretion within a single cell line.

Full Text

Duke Authors

Cited Authors

  • Gaisano, HY; Huang, X; Sheu, L; Ghai, M; Newgard, CB; Trinh, KY; Trimble, WS

Published Date

  • July 14, 1999

Published In

Volume / Issue

  • 260 / 3

Start / End Page

  • 781 - 784

PubMed ID

  • 10403842

International Standard Serial Number (ISSN)

  • 0006-291X

Digital Object Identifier (DOI)

  • 10.1006/bbrc.1999.0987

Language

  • eng

Conference Location

  • United States