Snare protein expression and adenoviral transfection of amphicrine AR42J.

Published

Journal Article

The amphicrine AR42J acinar cell line is an excellent model to study both exocrine and neuroendocrine exocytotic mechanisms. As a first step toward this goal, we determined the specific isoforms of the v- and t-SNARE and Munc18 families expressed in these cells. In addition, we show that dexamethasone-induced differentiation toward the exocrine phenotype causes an upregulation of several of these proteins. AR42J is notoriously difficult to transfect, limiting its usefulness as a model. However, we have now overcome this obstacle by acheiving high efficiency expression of a beta-galactosidase reporter gene and truncated SNAP-25 gene using adenoviral infection techniques. The AR42J cells can now be used to pursue and elucidate the distinct functions of individual SNARE isoforms used in endocrine and exocrine secretion within a single cell line.

Full Text

Duke Authors

Cited Authors

  • Gaisano, HY; Huang, X; Sheu, L; Ghai, M; Newgard, CB; Trinh, KY; Trimble, WS

Published Date

  • July 14, 1999

Published In

Volume / Issue

  • 260 / 3

Start / End Page

  • 781 - 784

PubMed ID

  • 10403842

Pubmed Central ID

  • 10403842

International Standard Serial Number (ISSN)

  • 0006-291X

Digital Object Identifier (DOI)

  • 10.1006/bbrc.1999.0987

Language

  • eng

Conference Location

  • United States