Fundamental metabolic differences between hepatocytes and islet beta-cells revealed by glucokinase overexpression.

Journal Article

Adenovirus-mediated overexpression of the glucose phosphorylating enzyme glucokinase causes large changes in glycolytic flux and glucose storage in isolated rat hepatocytes, but not in pancreatic islets. We have used the well-differentiated insulinoma cell line INS-1 to investigate the basis for these apparent cell-type specific differences. We find that 2- or 5-[3H]glucose usage is increased at low (

Full Text

Duke Authors

Cited Authors

  • Berman, HK; Newgard, CB

Published Date

  • March 31, 1998

Published In

Volume / Issue

  • 37 / 13

Start / End Page

  • 4543 - 4552

PubMed ID

  • 9521775

International Standard Serial Number (ISSN)

  • 0006-2960

Digital Object Identifier (DOI)

  • 10.1021/bi9726133

Language

  • eng

Conference Location

  • United States