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Expression of reg/PSP, a pancreatic exocrine gene: relationship to changes in islet beta-cell mass.

Publication ,  Journal Article
Miyaura, C; Chen, L; Appel, M; Alam, T; Inman, L; Hughes, SD; Milburn, JL; Unger, RH; Newgard, CB
Published in: Mol Endocrinol
February 1991

A cDNA termed reg was recently isolated by differential screening of a library prepared from regenerating islets isolated from pancreatic remnants of rats subjected to 90% pancreatectomy and nicotinamide treatment. This led to speculation that this gene may be involved in expansion of beta-cell mass. In the current study we have measured reg expression after implantation and resection of a solid insulinoma tumor into rats, maneuvers known, respectively, to reduce and reexpand the volume of beta-cells in the islet. Animals with an implanted insulinoma tumor became profoundly hypoglycemic. Islet beta-cells declined from the normal 75% of total islet volume to less than 30%, in concert with a marked reduction in the reg mRNA level. Removal of the tumor resulted in a sharp increase in beta-cell replication, as measured by [3H]thymidine incorporation and a return to normal beta-cell volume within 4 days of tumor resection. This was associated with a transient induction in reg expression compared to that in tumor-bearing animals, effectively returning the amount of reg mRNA to the levels found in normal animals within 48 h; at later time points after tumor removal (3-7 days) reg expression declined, but then rose toward normal. In situ hybridization analysis localized the initial induction in reg mRNA expression to the exocrine pancreas. Continuous infusion of insulin into normal rats for 4 days, a maneuver that does not significantly reduce beta-cell mass, resulted in dramatically reduced insulin mRNA in islets, but no change in the levels of reg mRNA. We conclude that the diminution in pancreatic beta-cell mass caused by subcutaneous implantation of an insulinoma is associated with reduced reg gene expression and that the increase in beta-cell replication after resection of the tumor is preceded by return of reg gene expression toward normal.

Duke Scholars

Published In

Mol Endocrinol

DOI

ISSN

0888-8809

Publication Date

February 1991

Volume

5

Issue

2

Start / End Page

226 / 234

Location

United States

Related Subject Headings

  • Regeneration
  • Rats, Inbred Strains
  • Rats
  • RNA, Messenger
  • Phosphoproteins
  • Pancreatic Neoplasms
  • Pancreas
  • Nucleic Acid Hybridization
  • Nerve Tissue Proteins
  • Neoplasm Transplantation
 

Citation

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MLA
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Miyaura, C., Chen, L., Appel, M., Alam, T., Inman, L., Hughes, S. D., … Newgard, C. B. (1991). Expression of reg/PSP, a pancreatic exocrine gene: relationship to changes in islet beta-cell mass. Mol Endocrinol, 5(2), 226–234. https://doi.org/10.1210/mend-5-2-226
Miyaura, C., L. Chen, M. Appel, T. Alam, L. Inman, S. D. Hughes, J. L. Milburn, R. H. Unger, and C. B. Newgard. “Expression of reg/PSP, a pancreatic exocrine gene: relationship to changes in islet beta-cell mass.Mol Endocrinol 5, no. 2 (February 1991): 226–34. https://doi.org/10.1210/mend-5-2-226.
Miyaura C, Chen L, Appel M, Alam T, Inman L, Hughes SD, et al. Expression of reg/PSP, a pancreatic exocrine gene: relationship to changes in islet beta-cell mass. Mol Endocrinol. 1991 Feb;5(2):226–34.
Miyaura, C., et al. “Expression of reg/PSP, a pancreatic exocrine gene: relationship to changes in islet beta-cell mass.Mol Endocrinol, vol. 5, no. 2, Feb. 1991, pp. 226–34. Pubmed, doi:10.1210/mend-5-2-226.
Miyaura C, Chen L, Appel M, Alam T, Inman L, Hughes SD, Milburn JL, Unger RH, Newgard CB. Expression of reg/PSP, a pancreatic exocrine gene: relationship to changes in islet beta-cell mass. Mol Endocrinol. 1991 Feb;5(2):226–234.

Published In

Mol Endocrinol

DOI

ISSN

0888-8809

Publication Date

February 1991

Volume

5

Issue

2

Start / End Page

226 / 234

Location

United States

Related Subject Headings

  • Regeneration
  • Rats, Inbred Strains
  • Rats
  • RNA, Messenger
  • Phosphoproteins
  • Pancreatic Neoplasms
  • Pancreas
  • Nucleic Acid Hybridization
  • Nerve Tissue Proteins
  • Neoplasm Transplantation