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The high Km glucose transporter of islets of Langerhans is functionally similar to the low affinity transporter of liver and has an identical primary sequence.

Publication ,  Journal Article
Johnson, JH; Newgard, CB; Milburn, JL; Lodish, HF; Thorens, B
Published in: J Biol Chem
April 25, 1990

The liver has been shown to contain a facilitated diffusion glucose transporter with high Km for glucose that is structurally distinct from the low Km glucose transporters found in most other tissues. We find that 3-O-methyl glucose is greater than 90% equilibrated across dispersed islet cells within 60 s, consistent with a facilitated diffusion transport mechanism. L-Glucose uptake was minimal throughout the time course, indicating stereospecificity. Measurement of glucose transport over a range of 3-O-methyl glucose concentrations from 0.05 to 60 mM revealed the presence of a component of glucose transport with an apparent Km of 17 mM, a value essentially identical to that previously reported for liver. Interestingly, a second component of glucose transport was also observed with an apparent Km of 1.4 mM, as has been reported for other tissues such as erythrocytes that are known to contain the "HepG2" or "erythroid/brain" type glucose transporter. Further evidence for the existence of two transport components is provided by the observation that a low concentration of cytochalasin B (0.4 microM) completely inhibits the low Km transport activity but has no effect on the high Km transporter. The kinetic similarity of high Km glucose transport in liver and islets is readily understood in light of our structural analysis. Sequence analysis of cDNA clones indicates that the liver and islet glucose transporters have identical sequences and, thus, are the products of the same gene.

Duke Scholars

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

April 25, 1990

Volume

265

Issue

12

Start / End Page

6548 / 6551

Location

United States

Related Subject Headings

  • Urea
  • Rats
  • Polymerase Chain Reaction
  • Nucleic Acid Hybridization
  • Monosaccharide Transport Proteins
  • Methylglucosides
  • Liver
  • Kinetics
  • Islets of Langerhans
  • Gene Library
 

Citation

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Johnson, J. H., Newgard, C. B., Milburn, J. L., Lodish, H. F., & Thorens, B. (1990). The high Km glucose transporter of islets of Langerhans is functionally similar to the low affinity transporter of liver and has an identical primary sequence. J Biol Chem, 265(12), 6548–6551.
Johnson, J. H., C. B. Newgard, J. L. Milburn, H. F. Lodish, and B. Thorens. “The high Km glucose transporter of islets of Langerhans is functionally similar to the low affinity transporter of liver and has an identical primary sequence.J Biol Chem 265, no. 12 (April 25, 1990): 6548–51.

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

April 25, 1990

Volume

265

Issue

12

Start / End Page

6548 / 6551

Location

United States

Related Subject Headings

  • Urea
  • Rats
  • Polymerase Chain Reaction
  • Nucleic Acid Hybridization
  • Monosaccharide Transport Proteins
  • Methylglucosides
  • Liver
  • Kinetics
  • Islets of Langerhans
  • Gene Library