Stimulus/secretion coupling factors in glucose-stimulated insulin secretion: insights gained from a multidisciplinary approach.

Journal Article (Journal Article;Review)

There is a growing appreciation for the complexity of the pathways involved in glucose-stimulated insulin secretion (GSIS) from pancreatic islet beta-cells. In our laboratory, this has stimulated the development of an interdisciplinary approach to the problem. In this study, we review recent studies combining the tools of recombinant adenovirus for gene delivery, the development of novel cell lines that exhibit either robust or weak GSIS, and nuclear magnetic resonance imaging for metabolic fingerprinting of glucose-stimulated cells. Using these tools, we demonstrate a potentially important role for pyruvate carboxylase-mediated pyruvate cycling pathways in the control of GSIS, and discuss potential coupling factors produced by such pathways.

Full Text

Duke Authors

Cited Authors

  • Newgard, CB; Lu, D; Jensen, MV; Schissler, J; Boucher, A; Burgess, S; Sherry, AD

Published Date

  • December 2002

Published In

Volume / Issue

  • 51 Suppl 3 /

Start / End Page

  • S389 - S393

PubMed ID

  • 12475781

Pubmed Central ID

  • 12475781

International Standard Serial Number (ISSN)

  • 0012-1797

Digital Object Identifier (DOI)

  • 10.2337/diabetes.51.2007.s389


  • eng

Conference Location

  • United States