Skip to main content
Journal cover image

Rat amylin: cloning and tissue-specific expression in pancreatic islets.

Publication ,  Journal Article
Leffert, JD; Newgard, CB; Okamoto, H; Milburn, JL; Luskey, KL
Published in: Proc Natl Acad Sci U S A
May 1989

Amyloid deposits in the islets of Langerhans of the pancreas are a common finding in non-insulin-dependent diabetes mellitus. The main protein constituent of these deposits is a 37-amino acid peptide known as amylin that resembles calcitonin gene-related peptide, a neuropeptide. We have isolated cDNA clones corresponding to the rat amylin precursor from an islet cDNA library and we show that this peptide is encoded in a 0.9-kilobase mRNA that is translated to yield a 93-amino acid precursor. The amylin peptide is bordered by dibasic residues, suggesting that it is proteolyzed like calcitonin gene-related peptide. The peptide sequences flanking the amylin sequence do not resemble the calcitonin gene-related peptide flanking sequences. RNA hybridization studies show that amylin mRNA is abundant in the islets of Langerhans but is not present in the brain or seven other tissues examined. Dietary changes, such as fasting or fasting and refeeding, have little effect on amylin mRNA expression. This tissue specificity suggests that amylin is involved in specific signaling pathways related to islet function.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

May 1989

Volume

86

Issue

9

Start / End Page

3127 / 3130

Location

United States

Related Subject Headings

  • Tissue Distribution
  • Sequence Homology, Nucleic Acid
  • Rats
  • RNA, Messenger
  • Protein Precursors
  • Protein Biosynthesis
  • Nucleic Acid Hybridization
  • Neuropeptides
  • Molecular Sequence Data
  • Islets of Langerhans
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Leffert, J. D., Newgard, C. B., Okamoto, H., Milburn, J. L., & Luskey, K. L. (1989). Rat amylin: cloning and tissue-specific expression in pancreatic islets. Proc Natl Acad Sci U S A, 86(9), 3127–3130. https://doi.org/10.1073/pnas.86.9.3127
Leffert, J. D., C. B. Newgard, H. Okamoto, J. L. Milburn, and K. L. Luskey. “Rat amylin: cloning and tissue-specific expression in pancreatic islets.Proc Natl Acad Sci U S A 86, no. 9 (May 1989): 3127–30. https://doi.org/10.1073/pnas.86.9.3127.
Leffert JD, Newgard CB, Okamoto H, Milburn JL, Luskey KL. Rat amylin: cloning and tissue-specific expression in pancreatic islets. Proc Natl Acad Sci U S A. 1989 May;86(9):3127–30.
Leffert, J. D., et al. “Rat amylin: cloning and tissue-specific expression in pancreatic islets.Proc Natl Acad Sci U S A, vol. 86, no. 9, May 1989, pp. 3127–30. Pubmed, doi:10.1073/pnas.86.9.3127.
Leffert JD, Newgard CB, Okamoto H, Milburn JL, Luskey KL. Rat amylin: cloning and tissue-specific expression in pancreatic islets. Proc Natl Acad Sci U S A. 1989 May;86(9):3127–3130.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

May 1989

Volume

86

Issue

9

Start / End Page

3127 / 3130

Location

United States

Related Subject Headings

  • Tissue Distribution
  • Sequence Homology, Nucleic Acid
  • Rats
  • RNA, Messenger
  • Protein Precursors
  • Protein Biosynthesis
  • Nucleic Acid Hybridization
  • Neuropeptides
  • Molecular Sequence Data
  • Islets of Langerhans