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Autoantibodies to the GLUT-2 glucose transporter of beta cells in insulin-dependent diabetes mellitus of recent onset.

Publication ,  Journal Article
Inman, LR; McAllister, CT; Chen, L; Hughes, S; Newgard, CB; Kettman, JR; Unger, RH; Johnson, JH
Published in: Proc Natl Acad Sci U S A
February 15, 1993

Purified immunoglobulin G (IgG) from the serum of patients with insulin-dependent diabetes mellitus (IDDM) of recent onset inhibits high-Km uptake of 3-O-methyl-beta-D-glucose by rat pancreatic islets. To determine if the inhibition is the result of antibodies against GLUT-2, the high-Km glucose transporter of beta cells, we incubated IDDM sera with rat islet cells and with AtT-20ins cells transfected to express GLUT-2. IDDM sera inhibited glucose uptake in islet cells and in GLUT-2-expressing AtT-20ins cells but not in AtT-20ins cells transfected to express the low-Km isoform, GLUT-1. In 24 of 30 (77%) patients with newly diagnosed IDDM, IgG binding as measured by immunofluorescence and flow cytometry of the cells transfected to express GLUT-2 was > 2 standard deviations from the mean of the nondiabetic population; 29 of 31 (96%) of nondiabetic children were negative (P < 0.0001). Increased IgG binding could be removed by absorption with GLUT-2-expressing cells but not with GLUT-1-expressing cells. We conclude that most patients with IDDM of recent onset have autoantibodies to GLUT-2.

Duke Scholars

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

February 15, 1993

Volume

90

Issue

4

Start / End Page

1281 / 1284

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Monosaccharide Transport Proteins
  • Methylglucosides
  • Male
  • Islets of Langerhans
  • Immunoglobulin G
  • Humans
  • Flow Cytometry
  • Female
  • Diabetes Mellitus, Type 1
 

Citation

APA
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ICMJE
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Inman, L. R., McAllister, C. T., Chen, L., Hughes, S., Newgard, C. B., Kettman, J. R., … Johnson, J. H. (1993). Autoantibodies to the GLUT-2 glucose transporter of beta cells in insulin-dependent diabetes mellitus of recent onset. Proc Natl Acad Sci U S A, 90(4), 1281–1284. https://doi.org/10.1073/pnas.90.4.1281
Inman, L. R., C. T. McAllister, L. Chen, S. Hughes, C. B. Newgard, J. R. Kettman, R. H. Unger, and J. H. Johnson. “Autoantibodies to the GLUT-2 glucose transporter of beta cells in insulin-dependent diabetes mellitus of recent onset.Proc Natl Acad Sci U S A 90, no. 4 (February 15, 1993): 1281–84. https://doi.org/10.1073/pnas.90.4.1281.
Inman LR, McAllister CT, Chen L, Hughes S, Newgard CB, Kettman JR, et al. Autoantibodies to the GLUT-2 glucose transporter of beta cells in insulin-dependent diabetes mellitus of recent onset. Proc Natl Acad Sci U S A. 1993 Feb 15;90(4):1281–4.
Inman, L. R., et al. “Autoantibodies to the GLUT-2 glucose transporter of beta cells in insulin-dependent diabetes mellitus of recent onset.Proc Natl Acad Sci U S A, vol. 90, no. 4, Feb. 1993, pp. 1281–84. Pubmed, doi:10.1073/pnas.90.4.1281.
Inman LR, McAllister CT, Chen L, Hughes S, Newgard CB, Kettman JR, Unger RH, Johnson JH. Autoantibodies to the GLUT-2 glucose transporter of beta cells in insulin-dependent diabetes mellitus of recent onset. Proc Natl Acad Sci U S A. 1993 Feb 15;90(4):1281–1284.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

February 15, 1993

Volume

90

Issue

4

Start / End Page

1281 / 1284

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Monosaccharide Transport Proteins
  • Methylglucosides
  • Male
  • Islets of Langerhans
  • Immunoglobulin G
  • Humans
  • Flow Cytometry
  • Female
  • Diabetes Mellitus, Type 1