Disappearance of body fat in normal rats induced by adenovirus-mediated leptin gene therapy.


Journal Article

Sustained hyperleptinemia of 8 ng/ml was induced for 28 days in normal Wistar rats by infusing a recombinant adenovirus containing the rat leptin cDNA (AdCMV-leptin). Hyperleptinemic rats exhibited a 30-50% reduction in food intake and gained only 22 g over the experimental period versus 115-132 g in control animals that received saline infusions or a recombinant virus containing the beta-galactosidase gene (AdCMV-beta Gal). Body fat was absent in hyperleptinemic rats, whereas control rats pair-fed to the hyperleptinemic rats retained approximately 50% body fat. Further, plasma triglycerides and insulin levels were significantly lower in hyperleptinemic versus pair-fed controls, while fatty acid and glucose levels were similar in the two groups, suggestive of enhanced insulin sensitivity in the hyperleptinemic animals. Thus, despite equivalent reductions in food intake and weight gain in hyperleptinemic and pair-fed animals, identifiable fat tissue was completely ablated only in the former group, raising the possibility of a specific lipoatrophic activity for leptin.

Full Text

Duke Authors

Cited Authors

  • Chen, G; Koyama, K; Yuan, X; Lee, Y; Zhou, YT; O'Doherty, R; Newgard, CB; Unger, RH

Published Date

  • December 10, 1996

Published In

Volume / Issue

  • 93 / 25

Start / End Page

  • 14795 - 14799

PubMed ID

  • 8962134

Pubmed Central ID

  • 8962134

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.93.25.14795


  • eng

Conference Location

  • United States