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Analysis of the protein products encoded by variant glucokinase transcripts via expression in bacteria.

Publication ,  Journal Article
Quaade, C; Hughes, SD; Coats, WS; Sestak, AL; Iynedjian, PB; Newgard, CB
Published in: FEBS Lett
March 11, 1991

Five variant transcripts of the single rat glucokinase gene have been described that are naturally expressed in islets of Langerhans, liver and anterior pituitary. Four of these were prepared as cDNA and expressed in bacteria in order to begin to address their physiological roles. Expression of constructs pGKB1 (normal islet/pituitary glucokinase) and pGKL1 (normal liver glucokinase) resulted in a glucose-dependent, glucokinase-like activity, 7-fold and 45-fold, respectively, above background. Expression of pGKB3 (variant islet/pituitary glucokinase) and pGKL2 (variant liver glucokinase) in contrast, did not result in any glucokinase-like activity.

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Published In

FEBS Lett

DOI

ISSN

0014-5793

Publication Date

March 11, 1991

Volume

280

Issue

1

Start / End Page

47 / 52

Location

England

Related Subject Headings

  • Rats
  • RNA, Messenger
  • Pituitary Gland, Anterior
  • Phosphorylation
  • Molecular Sequence Data
  • Isomerism
  • Islets of Langerhans
  • Glucose
  • Glucokinase
  • Genetic Variation
 

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Quaade, C., Hughes, S. D., Coats, W. S., Sestak, A. L., Iynedjian, P. B., & Newgard, C. B. (1991). Analysis of the protein products encoded by variant glucokinase transcripts via expression in bacteria. FEBS Lett, 280(1), 47–52. https://doi.org/10.1016/0014-5793(91)80201-d
Quaade, C., S. D. Hughes, W. S. Coats, A. L. Sestak, P. B. Iynedjian, and C. B. Newgard. “Analysis of the protein products encoded by variant glucokinase transcripts via expression in bacteria.FEBS Lett 280, no. 1 (March 11, 1991): 47–52. https://doi.org/10.1016/0014-5793(91)80201-d.
Quaade C, Hughes SD, Coats WS, Sestak AL, Iynedjian PB, Newgard CB. Analysis of the protein products encoded by variant glucokinase transcripts via expression in bacteria. FEBS Lett. 1991 Mar 11;280(1):47–52.
Quaade, C., et al. “Analysis of the protein products encoded by variant glucokinase transcripts via expression in bacteria.FEBS Lett, vol. 280, no. 1, Mar. 1991, pp. 47–52. Pubmed, doi:10.1016/0014-5793(91)80201-d.
Quaade C, Hughes SD, Coats WS, Sestak AL, Iynedjian PB, Newgard CB. Analysis of the protein products encoded by variant glucokinase transcripts via expression in bacteria. FEBS Lett. 1991 Mar 11;280(1):47–52.
Journal cover image

Published In

FEBS Lett

DOI

ISSN

0014-5793

Publication Date

March 11, 1991

Volume

280

Issue

1

Start / End Page

47 / 52

Location

England

Related Subject Headings

  • Rats
  • RNA, Messenger
  • Pituitary Gland, Anterior
  • Phosphorylation
  • Molecular Sequence Data
  • Isomerism
  • Islets of Langerhans
  • Glucose
  • Glucokinase
  • Genetic Variation