Analysis of the protein products encoded by variant glucokinase transcripts via expression in bacteria.

Journal Article

Five variant transcripts of the single rat glucokinase gene have been described that are naturally expressed in islets of Langerhans, liver and anterior pituitary. Four of these were prepared as cDNA and expressed in bacteria in order to begin to address their physiological roles. Expression of constructs pGKB1 (normal islet/pituitary glucokinase) and pGKL1 (normal liver glucokinase) resulted in a glucose-dependent, glucokinase-like activity, 7-fold and 45-fold, respectively, above background. Expression of pGKB3 (variant islet/pituitary glucokinase) and pGKL2 (variant liver glucokinase) in contrast, did not result in any glucokinase-like activity.

Full Text

Duke Authors

Cited Authors

  • Quaade, C; Hughes, SD; Coats, WS; Sestak, AL; Iynedjian, PB; Newgard, CB

Published Date

  • March 11, 1991

Published In

Volume / Issue

  • 280 / 1

Start / End Page

  • 47 - 52

PubMed ID

  • 2009966

International Standard Serial Number (ISSN)

  • 0014-5793

Language

  • eng

Conference Location

  • England