Skip to main content
Journal cover image

Filamentous phage IKe mRNAs conserve form and function despite divergence in regulatory elements.

Publication ,  Journal Article
Stump, MD; Madison-Antenucci, S; Kokoska, RJ; Steege, DA
Published in: J Mol Biol
February 14, 1997

As a means of determining whether there has been selection to conserve the basic pattern of filamentous phage mRNAs, the major mRNAs representing genes II to VIII have been defined for a phage distantly related to the Ff group specific for Escherichia coli hosts bearing F pili. Phage IKe has a genome with 55% identity with the Ff genome and infects E. coli strains bearing N pili. The results reveal a remarkably similar pattern of overlapping polycistronic mRNAs with a common 3' end and unique 5' ends. The IKe mRNAs, like the Ff phage mRNAs, represent a combination of primary transcripts and processed RNAs. However, examination of the sequences containing the RNA endpoint positions revealed that effectively the only highly conserved regulatory element is the rho-independent terminator that generates the common 3' end. Promoters and processing sites have not been maintained in identical positions, but frequently are placed so as to yield RNAs with similar coding function. By conserving the pattern of transcription and processing despite divergence in the regulatory elements and possibly the requirements for host, endoribonucleases, the results argue that the pattern is not simply fortuitous.

Published In

J Mol Biol

DOI

ISSN

0022-2836

Publication Date

February 14, 1997

Volume

266

Issue

1

Start / End Page

51 / 65

Location

Netherlands

Related Subject Headings

  • Transcription, Genetic
  • Terminator Regions, Genetic
  • Sequence Homology, Nucleic Acid
  • Regulatory Sequences, Nucleic Acid
  • RNA, Messenger
  • Promoter Regions, Genetic
  • Molecular Sequence Data
  • Inovirus
  • Genome, Viral
  • Genetic Variation
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Stump, M. D., Madison-Antenucci, S., Kokoska, R. J., & Steege, D. A. (1997). Filamentous phage IKe mRNAs conserve form and function despite divergence in regulatory elements. J Mol Biol, 266(1), 51–65. https://doi.org/10.1006/jmbi.1996.0766
Stump, M. D., S. Madison-Antenucci, R. J. Kokoska, and D. A. Steege. “Filamentous phage IKe mRNAs conserve form and function despite divergence in regulatory elements.J Mol Biol 266, no. 1 (February 14, 1997): 51–65. https://doi.org/10.1006/jmbi.1996.0766.
Stump MD, Madison-Antenucci S, Kokoska RJ, Steege DA. Filamentous phage IKe mRNAs conserve form and function despite divergence in regulatory elements. J Mol Biol. 1997 Feb 14;266(1):51–65.
Stump, M. D., et al. “Filamentous phage IKe mRNAs conserve form and function despite divergence in regulatory elements.J Mol Biol, vol. 266, no. 1, Feb. 1997, pp. 51–65. Pubmed, doi:10.1006/jmbi.1996.0766.
Stump MD, Madison-Antenucci S, Kokoska RJ, Steege DA. Filamentous phage IKe mRNAs conserve form and function despite divergence in regulatory elements. J Mol Biol. 1997 Feb 14;266(1):51–65.
Journal cover image

Published In

J Mol Biol

DOI

ISSN

0022-2836

Publication Date

February 14, 1997

Volume

266

Issue

1

Start / End Page

51 / 65

Location

Netherlands

Related Subject Headings

  • Transcription, Genetic
  • Terminator Regions, Genetic
  • Sequence Homology, Nucleic Acid
  • Regulatory Sequences, Nucleic Acid
  • RNA, Messenger
  • Promoter Regions, Genetic
  • Molecular Sequence Data
  • Inovirus
  • Genome, Viral
  • Genetic Variation