Isolation and characterization of lambda transducing bacteriophages for the su1+ (supD minus) amber suppressor of Escherichia coli.


Journal Article

Specialized lambda transducing phages for the sul+ (supD-) amber suppressor in Escherichia coli K-12 have been isolated, using a secondary site lambda-cI857 lysogen in which we have shown the prophage to be closely linked to sul+.sul+ transducing particles were detected frequently, at 10-5 per plaque-forming unit, in lysates prepared from the secondary-site lysogens. High-frequency transducing lysates were obtained from several independently isolated sul+ transductants and were analyzed by CsCl equilibrium density gradient centrifugation. The transducing phages are defective; marker rescue analysis indicates that the lambda-N gene is not present. In lambda-cI857DELTANdSul+, a bio-type transducing phage, the genes specifying recombination and excision functions have been replaced by bacterial deoxyribonucleic acid.

Full Text

Cited Authors

  • Steege, DA; Low, B

Published Date

  • April 1, 1975

Published In

Volume / Issue

  • 122 / 1

Start / End Page

  • 120 - 128

PubMed ID

  • 1091618

Pubmed Central ID

  • 1091618

International Standard Serial Number (ISSN)

  • 0021-9193


  • eng

Conference Location

  • United States