Phage fl mRNA processing in Escherichia coli: search for the upstream products of endonuclease cleavage, requirement for the product of the altered mRNA stability (ams) locus.


Journal Article

In Escherichia coli infected with the filamentous phage f1, a number of the polycistronic phage mRNA species are generated through post-transcriptional processing by host nuclease activity. In this paper we review experimental evidence assessing whether known RNases are involved in mediating these processing events, and we use S1 nuclease mapping methods to visualize putative upstream products of endonuclease cleavage. By examining f1 processing in a phage-infected host bearing a temperature-sensitive allele of the altered message stability locus (ams), we show that production of the major processed species requires a component of the host cell which functions in the messenger RNA decay process.

Full Text

Cited Authors

  • Kokoska, RJ; Blumer, KJ; Steege, DA

Published Date

  • November 1990

Published In

Volume / Issue

  • 72 / 11

Start / End Page

  • 803 - 811

PubMed ID

  • 2085543

Pubmed Central ID

  • 2085543

International Standard Serial Number (ISSN)

  • 0300-9084

Digital Object Identifier (DOI)

  • 10.1016/0300-9084(90)90189-n


  • eng

Conference Location

  • France