Mechanism of activation of human plasminogen by the activator complex, streptokinase-plasmin.

Published

Journal Article

The object of this investigation was to distinguish between two potential mechanisms of activation of human plasminogen (HPg) to plasmin (HPm) by catalytic levels of the activator complex, streptokinase.plasmin (SK.HPm). One mechanism, which is widely supported, postulates an enzymatic role for SK.HPm in the conversion of molar excesses of plasminogen to plasmin. A more recently described kinetic mechanism involves a direct conversion of HPg to HPm by streptokinase (SK). Here, it is believed that displacement of HPm from SK.HPm by excess HPg is the major source of free HPm in the activation process. The present paper shows that SK is not capable of undergoing rapid exchange from SK.HPm to other HPg or HPm molecules, thus precluding the possibility of direct activation of HPg by SK. Our evidence supports a mechanism involving an enzymatic role for SK.HPm as the major means of converting free HPg to HPm.

Full Text

Duke Authors

Cited Authors

  • Gonzalez-Gronow, M; Siefring, GE; Castellino, FJ

Published Date

  • February 25, 1978

Published In

Volume / Issue

  • 253 / 4

Start / End Page

  • 1090 - 1094

PubMed ID

  • 146709

Pubmed Central ID

  • 146709

International Standard Serial Number (ISSN)

  • 0021-9258

Language

  • eng

Conference Location

  • United States