Efficient sporulation of yeast in media buffered near pH6.
Journal Article
Diploid cells of Saccharomyces cerevisiae underwent meiosis and sporulation when placed in 1% potassium acetate sporulation medium. In unbuffered sporulation medium the pH rose very rapidly, reaching pH 8.4 after 2 h of sporulation. Under these conditions, the uptake of radioactive adenine and lysine was extremely limited, and ascus formation was insensitive to inhibitors such as 5-fluorouracil and canavanine. By using several different buffers, we showed that an increase in the pH of sporulation media was not necessary for sporulation to occur. Spore viability and the kinetics of ascus and prototroph formation were normal for cells sporulated in several types of media buffered as low as pH 5.5. Incubation of sporulating cells below pH 6.5 did cause separation of small but viable buds from their mother cells. With sporulating cells buffered below pH 6.5, the incorporation of radioactive adenine and lysine was greatly enhanced and cells became sensitive to inhibition by 5-fluorouracil and canavanine.
Full Text
Duke Authors
Cited Authors
- McCusker, JH; Haber, JE
Published Date
- October 1, 1977
Published In
Volume / Issue
- 132 / 1
Start / End Page
- 180 - 185
PubMed ID
- 21160
Pubmed Central ID
- 21160
International Standard Serial Number (ISSN)
- 0021-9193
Digital Object Identifier (DOI)
- 10.1128/JB.132.1.180-185.1977
Language
- eng
Conference Location
- United States