Genomic mismatch scanning: a new approach to genetic linkage mapping.

Journal Article (Journal Article)

Genomic mismatch scanning (GMS) is a new method of genetic linkage analysis that does not require conventional polymorphic markers or gel electrophoresis. GMS is ideally suited to affected-relative-pair mapping. DNA fragments from all regions of identity-by-descent between two relatives are isolated based on their ability to form extensive mismatch-free hybrid molecules. The genomic origin of this selected pool of DNA fragments is then mapped in a single hybridization step. Here we demonstrate the practicality of GMS in a model organism, Saccharomyces cerevisiae. GMS is likely to be applicable to other organisms, including humans, and may be of particular value in mapping complex genetic traits.

Full Text

Duke Authors

Cited Authors

  • Nelson, SF; McCusker, JH; Sander, MA; Kee, Y; Modrich, P; Brown, PO

Published Date

  • May 1993

Published In

Volume / Issue

  • 4 / 1

Start / End Page

  • 11 - 18

PubMed ID

  • 8513319

International Standard Serial Number (ISSN)

  • 1061-4036

Digital Object Identifier (DOI)

  • 10.1038/ng0593-11


  • eng

Conference Location

  • United States