Genomic mismatch scanning: a new approach to genetic linkage mapping.
Publication
, Journal Article
Nelson, SF; McCusker, JH; Sander, MA; Kee, Y; Modrich, P; Brown, PO
Published in: Nat Genet
May 1993
Genomic mismatch scanning (GMS) is a new method of genetic linkage analysis that does not require conventional polymorphic markers or gel electrophoresis. GMS is ideally suited to affected-relative-pair mapping. DNA fragments from all regions of identity-by-descent between two relatives are isolated based on their ability to form extensive mismatch-free hybrid molecules. The genomic origin of this selected pool of DNA fragments is then mapped in a single hybridization step. Here we demonstrate the practicality of GMS in a model organism, Saccharomyces cerevisiae. GMS is likely to be applicable to other organisms, including humans, and may be of particular value in mapping complex genetic traits.
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Published In
Nat Genet
DOI
ISSN
1061-4036
Publication Date
May 1993
Volume
4
Issue
1
Start / End Page
11 / 18
Location
United States
Related Subject Headings
- Site-Specific DNA-Methyltransferase (Adenine-Specific)
- Sensitivity and Specificity
- Saccharomyces cerevisiae
- Polymorphism, Genetic
- Nucleic Acid Hybridization
- Methyltransferases
- Methylation
- Humans
- Genome, Human
- Genome, Fungal
Citation
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Nelson, S. F., McCusker, J. H., Sander, M. A., Kee, Y., Modrich, P., & Brown, P. O. (1993). Genomic mismatch scanning: a new approach to genetic linkage mapping. Nat Genet, 4(1), 11–18. https://doi.org/10.1038/ng0593-11
Nelson, S. F., J. H. McCusker, M. A. Sander, Y. Kee, P. Modrich, and P. O. Brown. “Genomic mismatch scanning: a new approach to genetic linkage mapping.” Nat Genet 4, no. 1 (May 1993): 11–18. https://doi.org/10.1038/ng0593-11.
Nelson SF, McCusker JH, Sander MA, Kee Y, Modrich P, Brown PO. Genomic mismatch scanning: a new approach to genetic linkage mapping. Nat Genet. 1993 May;4(1):11–8.
Nelson, S. F., et al. “Genomic mismatch scanning: a new approach to genetic linkage mapping.” Nat Genet, vol. 4, no. 1, May 1993, pp. 11–18. Pubmed, doi:10.1038/ng0593-11.
Nelson SF, McCusker JH, Sander MA, Kee Y, Modrich P, Brown PO. Genomic mismatch scanning: a new approach to genetic linkage mapping. Nat Genet. 1993 May;4(1):11–18.
Published In
Nat Genet
DOI
ISSN
1061-4036
Publication Date
May 1993
Volume
4
Issue
1
Start / End Page
11 / 18
Location
United States
Related Subject Headings
- Site-Specific DNA-Methyltransferase (Adenine-Specific)
- Sensitivity and Specificity
- Saccharomyces cerevisiae
- Polymorphism, Genetic
- Nucleic Acid Hybridization
- Methyltransferases
- Methylation
- Humans
- Genome, Human
- Genome, Fungal