Genomic mismatch scanning: a new approach to genetic linkage mapping.
Journal Article (Journal Article)
Genomic mismatch scanning (GMS) is a new method of genetic linkage analysis that does not require conventional polymorphic markers or gel electrophoresis. GMS is ideally suited to affected-relative-pair mapping. DNA fragments from all regions of identity-by-descent between two relatives are isolated based on their ability to form extensive mismatch-free hybrid molecules. The genomic origin of this selected pool of DNA fragments is then mapped in a single hybridization step. Here we demonstrate the practicality of GMS in a model organism, Saccharomyces cerevisiae. GMS is likely to be applicable to other organisms, including humans, and may be of particular value in mapping complex genetic traits.
- Nelson, SF; McCusker, JH; Sander, MA; Kee, Y; Modrich, P; Brown, PO
- May 1993
Volume / Issue
- 4 / 1
Start / End Page
- 11 - 18
International Standard Serial Number (ISSN)
Digital Object Identifier (DOI)
- United States