Yeast microarrays for genome wide parallel genetic and gene expression analysis.

Journal Article

We have developed high-density DNA microarrays of yeast ORFs. These microarrays can monitor hybridization to ORFs for applications such as quantitative differential gene expression analysis and screening for sequence polymorphisms. Automated scripts retrieved sequence information from public databases to locate predicted ORFs and select appropriate primers for amplification. The primers were used to amplify yeast ORFs in 96-well plates, and the resulting products were arrayed using an automated micro arraying device. Arrays containing up to 2,479 yeast ORFs were printed on a single slide. The hybridization of fluorescently labeled samples to the array were detected and quantitated with a laser confocal scanning microscope. Applications of the microarrays are shown for genetic and gene expression analysis at the whole genome level.

Full Text

Duke Authors

Cited Authors

  • Lashkari, DA; DeRisi, JL; McCusker, JH; Namath, AF; Gentile, C; Hwang, SY; Brown, PO; Davis, RW

Published Date

  • November 25, 1997

Published In

Volume / Issue

  • 94 / 24

Start / End Page

  • 13057 - 13062

PubMed ID

  • 9371799

International Standard Serial Number (ISSN)

  • 0027-8424

Language

  • eng

Conference Location

  • United States