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Yeast microarrays for genome wide parallel genetic and gene expression analysis.

Publication ,  Journal Article
Lashkari, DA; DeRisi, JL; McCusker, JH; Namath, AF; Gentile, C; Hwang, SY; Brown, PO; Davis, RW
Published in: Proc Natl Acad Sci U S A
November 25, 1997
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We have developed high-density DNA microarrays of yeast ORFs. These microarrays can monitor hybridization to ORFs for applications such as quantitative differential gene expression analysis and screening for sequence polymorphisms. Automated scripts retrieved sequence information from public databases to locate predicted ORFs and select appropriate primers for amplification. The primers were used to amplify yeast ORFs in 96-well plates, and the resulting products were arrayed using an automated micro arraying device. Arrays containing up to 2,479 yeast ORFs were printed on a single slide. The hybridization of fluorescently labeled samples to the array were detected and quantitated with a laser confocal scanning microscope. Applications of the microarrays are shown for genetic and gene expression analysis at the whole genome level.

Duke Scholars

John Henry McCusker
Author John Henry McCusker Molecular Genetics and Microbiology
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Published In

Proc Natl Acad Sci U S A

DOI

10.1073/pnas.94.24.13057

ISSN

0027-8424

Publication Date

November 25, 1997

Volume

94

Issue

24

Start / End Page

13057 / 13062

Location

United States

Related Subject Headings

  • Saccharomyces cerevisiae
  • Open Reading Frames
  • Heat-Shock Response
  • Glucose
  • Genotype
  • Genome, Fungal
  • Gene Expression
  • Galactose
  • DNA, Complementary
  • DNA Primers
 

Citation

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Lashkari, D. A., DeRisi, J. L., McCusker, J. H., Namath, A. F., Gentile, C., Hwang, S. Y., … Davis, R. W. (1997). Yeast microarrays for genome wide parallel genetic and gene expression analysis. Proc Natl Acad Sci U S A, 94(24), 13057–13062. https://doi.org/10.1073/pnas.94.24.13057
Lashkari, D. A., J. L. DeRisi, J. H. McCusker, A. F. Namath, C. Gentile, S. Y. Hwang, P. O. Brown, and R. W. Davis. “Yeast microarrays for genome wide parallel genetic and gene expression analysis.” Proc Natl Acad Sci U S A 94, no. 24 (November 25, 1997): 13057–62. https://doi.org/10.1073/pnas.94.24.13057.
Lashkari DA, DeRisi JL, McCusker JH, Namath AF, Gentile C, Hwang SY, et al. Yeast microarrays for genome wide parallel genetic and gene expression analysis. Proc Natl Acad Sci U S A. 1997 Nov 25;94(24):13057–62.
Lashkari, D. A., et al. “Yeast microarrays for genome wide parallel genetic and gene expression analysis.” Proc Natl Acad Sci U S A, vol. 94, no. 24, Nov. 1997, pp. 13057–62. Pubmed, doi:10.1073/pnas.94.24.13057.
Lashkari DA, DeRisi JL, McCusker JH, Namath AF, Gentile C, Hwang SY, Brown PO, Davis RW. Yeast microarrays for genome wide parallel genetic and gene expression analysis. Proc Natl Acad Sci U S A. 1997 Nov 25;94(24):13057–13062.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

10.1073/pnas.94.24.13057

ISSN

0027-8424

Publication Date

November 25, 1997

Volume

94

Issue

24

Start / End Page

13057 / 13062

Location

United States

Related Subject Headings

  • Saccharomyces cerevisiae
  • Open Reading Frames
  • Heat-Shock Response
  • Glucose
  • Genotype
  • Genome, Fungal
  • Gene Expression
  • Galactose
  • DNA, Complementary
  • DNA Primers