Direct allelic variation scanning of the yeast genome.

Published

Journal Article

As more genomes are sequenced, the identification and characterization of the causes of heritable variation within a species will be increasingly important. It is demonstrated that allelic variation in any two isolates of a species can be scanned, mapped, and scored directly and efficiently without allele-specific polymerase chain reaction, without creating new strains or constructs, and without knowing the specific nature of the variation. A total of 3714 biallelic markers, spaced about every 3.5 kilobases, were identified by analyzing the patterns obtained when total genomic DNA from two different strains of yeast was hybridized to high-density oligonucleotide arrays. The markers were then used to simultaneously map a multidrug-resistance locus and four other loci with high resolution (11 to 64 kilobases).

Full Text

Duke Authors

Cited Authors

  • Winzeler, EA; Richards, DR; Conway, AR; Goldstein, AL; Kalman, S; McCullough, MJ; McCusker, JH; Stevens, DA; Wodicka, L; Lockhart, DJ; Davis, RW

Published Date

  • August 1998

Published In

Volume / Issue

  • 281 / 5380

Start / End Page

  • 1194 - 1197

PubMed ID

  • 9712584

Pubmed Central ID

  • 9712584

Electronic International Standard Serial Number (EISSN)

  • 1095-9203

International Standard Serial Number (ISSN)

  • 0036-8075

Digital Object Identifier (DOI)

  • 10.1126/science.281.5380.1194

Language

  • eng