Dissecting the architecture of a quantitative trait locus in yeast.

Journal Article (Journal Article)

Most phenotypic diversity in natural populations is characterized by differences in degree rather than in kind. Identification of the actual genes underlying these quantitative traits has proved difficult. As a result, little is known about their genetic architecture. The failures are thought to be due to the different contributions of many underlying genes to the phenotype and the ability of different combinations of genes and environmental factors to produce similar phenotypes. This study combined genome-wide mapping and a new genetic technique named reciprocal-hemizygosity analysis to achieve the complete dissection of a quantitative trait locus (QTL) in Saccharomyces cerevisiae. A QTL architecture was uncovered that was more complex than expected. Functional linkages both in cis and in trans were found between three tightly linked quantitative trait genes that are neither necessary nor sufficient in isolation. This arrangement of alleles explains heterosis (hybrid vigour), the increased fitness of the heterozygote compared with homozygotes. It also demonstrates a deficiency in current approaches to QTL dissection with implications extending to traits in other organisms, including human genetic diseases.

Full Text

Duke Authors

Cited Authors

  • Steinmetz, LM; Sinha, H; Richards, DR; Spiegelman, JI; Oefner, PJ; McCusker, JH; Davis, RW

Published Date

  • March 21, 2002

Published In

Volume / Issue

  • 416 / 6878

Start / End Page

  • 326 - 330

PubMed ID

  • 11907579

International Standard Serial Number (ISSN)

  • 0028-0836

Digital Object Identifier (DOI)

  • 10.1038/416326a


  • eng

Conference Location

  • England