Whole genome genetic-typing in yeast using high-density oligonucleotide arrays.

Journal Article (Journal Article)

Genome sequence information in combination with new technologies has allowed researchers to approach genetic problems in new ways. High-density oligonucleotide arrays were used to probe the genome content of the yeast Saccharomyces cerevisiae. We show that these arrays, containing oligonucleotides complementary to the sequenced strain of S. cerevisiae, can be used to identify open reading frames that are missing or present in higher or lower copy number in related isolates of S. cerevisiae. We apply this method to the characterization of the genome of a strain derived from a clinical isolate of S. cerevisiae. Our results show that the telomeres are the regions with the most variability between the two strains.

Full Text

Duke Authors

Cited Authors

  • Winzeler, EA; Lee, B; McCusker, JH; Davis, RW

Published Date

  • 1999

Published In

Volume / Issue

  • 118 Suppl /

Start / End Page

  • S73 - S80

PubMed ID

  • 10466139

International Standard Serial Number (ISSN)

  • 0031-1820

Digital Object Identifier (DOI)

  • 10.1017/s0031182099004047


  • eng

Conference Location

  • England