Whole genome analysis: experimental access to all genome sequenced segments through larger-scale efficient oligonucleotide synthesis and PCR.

Published

Journal Article

The recent ability to sequence whole genomes allows ready access to all genetic material. The approaches outlined here allow automated analysis of sequence for the synthesis of optimal primers in an automated multiplex oligonucleotide synthesizer (AMOS). The efficiency is such that all ORFs for an organism can be amplified by PCR. The resulting amplicons can be used directly in the construction of DNA arrays or can be cloned for a large variety of functional analyses. These tools allow a replacement of single-gene analysis with a highly efficient whole-genome analysis.

Full Text

Duke Authors

Cited Authors

  • Lashkari, DA; McCusker, JH; Davis, RW

Published Date

  • August 19, 1997

Published In

Volume / Issue

  • 94 / 17

Start / End Page

  • 8945 - 8947

PubMed ID

  • 9256414

Pubmed Central ID

  • 9256414

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.94.17.8945

Language

  • eng

Conference Location

  • United States