The suppressor gene scl1+ of Saccharomyces cerevisiae is essential for growth.
In Saccharomyces cerevisiae, the SCL-1 mutation is a dominant suppressor of the cycloheximide-resistant, temperature-sensitive (ts) lethal mutation, crl3 [McCusker and Haber, Genetics 119 (1988a) 303-315]. The wild-type scl1+ gene was isolated by screening subclones of the 35-kb region between TRP5 and LEU1 for restoration of the ts phenotype in an SCL1-1 crl3-2 strain. The scl1+ mRNA is about 900 nt long and encodes an open reading frame of 810 bp. The polypeptide deduced from scl1+ possesses a putative secretory signal peptide. The 5'-noncoding region may be under multiple controls, since it contains significant homology to the consensus sequences for the DNA-binding proteins, GCN4, GFI and, possibly, TUF. Gene disruption of scl1+ demonstrates that it is an essential gene.
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- Transformation, Genetic
- Suppression, Genetic
- Saccharomyces cerevisiae
- Restriction Mapping
- RNA, Fungal
- Plasmids
- Mutation
- Molecular Sequence Data
- Genes, Lethal
- Genes, Fungal
Citation
Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Transformation, Genetic
- Suppression, Genetic
- Saccharomyces cerevisiae
- Restriction Mapping
- RNA, Fungal
- Plasmids
- Mutation
- Molecular Sequence Data
- Genes, Lethal
- Genes, Fungal