The suppressor gene scl1+ of Saccharomyces cerevisiae is essential for growth.

Published

Journal Article

In Saccharomyces cerevisiae, the SCL-1 mutation is a dominant suppressor of the cycloheximide-resistant, temperature-sensitive (ts) lethal mutation, crl3 [McCusker and Haber, Genetics 119 (1988a) 303-315]. The wild-type scl1+ gene was isolated by screening subclones of the 35-kb region between TRP5 and LEU1 for restoration of the ts phenotype in an SCL1-1 crl3-2 strain. The scl1+ mRNA is about 900 nt long and encodes an open reading frame of 810 bp. The polypeptide deduced from scl1+ possesses a putative secretory signal peptide. The 5'-noncoding region may be under multiple controls, since it contains significant homology to the consensus sequences for the DNA-binding proteins, GCN4, GFI and, possibly, TUF. Gene disruption of scl1+ demonstrates that it is an essential gene.

Full Text

Duke Authors

Cited Authors

  • Balzi, E; Chen, WN; Capieaux, E; McCusker, JH; Haber, JE; Goffeau, A

Published Date

  • November 30, 1989

Published In

Volume / Issue

  • 83 / 2

Start / End Page

  • 271 - 279

PubMed ID

  • 2684789

Pubmed Central ID

  • 2684789

International Standard Serial Number (ISSN)

  • 0378-1119

Digital Object Identifier (DOI)

  • 10.1016/0378-1119(89)90113-3

Language

  • eng

Conference Location

  • Netherlands