A quantitative fluorescent method for measurement of bacterial adherence and phagocytosis.


Journal Article

We have developed a new two-color fluorescent method for the quantitative measurement of adherence and ingestion of Streptococcus pneumoniae by human monocytes. The method employs a fluorescent naphthalimide, Lucifer Yellow VS, that has been covalently linked to the bacterial cell wall. Bacteria were opsonized and allowed to adhere to monocytes. Lucifer Yellow did not alter the bacterial interaction with complement in serum or with the phagocytic cell. The ability of monocytes to ingest the adherent bacteria was tested under a variety of conditions. Rabbit antibody to Lucifer Yellow derivatized with Texas Red was used to detect monocyte-bound, but uningested bacteria. Dual laser flow cytometry simultaneously quantitated the total number of monocyte-associated S. pneumoniae and the number that remained surface adherent. This method allows separate analysis of the opsonins and receptors involved in bacterial adherence to phagocytes and in the ingestion process.

Full Text

Cited Authors

  • Sveum, RJ; Chused, TM; Frank, MM; Brown, EJ

Published Date

  • June 24, 1986

Published In

Volume / Issue

  • 90 / 2

Start / End Page

  • 257 - 264

PubMed ID

  • 3722828

Pubmed Central ID

  • 3722828

International Standard Serial Number (ISSN)

  • 0022-1759

Digital Object Identifier (DOI)

  • 10.1016/0022-1759(86)90083-9


  • eng

Conference Location

  • Netherlands