Two distinct genes encode small isoproteolipids affecting plasma membrane H(+)-ATPase activity of Saccharomyces cerevisiae.
Journal Article (Journal Article)
A small proteolipid called PMP1 is associated with yeast plasma membrane H(+)-ATPase (Navarre, C., Ghislain, M., Leterme, S., Ferroud, C., Dufour, J.-P., and Goffeau, A. (1992) J. Biol. Chem. 267, 6425-6428). We have identified a second Saccharomyces cerevisiae plasma membrane proteolipid gene by hybridization with a PMP1 probe. The sequence of the corresponding gene, called PMP2, is 92% identical to the PMP1 gene sequence. PMP2 encodes a 43-amino acid polypeptide that can be extracted from the membrane with chloroform/methanol. The two proteolipids differ at residue 21, which is an alanine in PMP1 and a serine in PMP2. The two PMP genes are similarly expressed in the wild-type strain, and no modification of the level of transcription of one PMP gene is detected in a strain deleted of the other. A regulatory function of the proteolipids is indicated by the observation that a strain lacking both PMP genes and no longer containing plasma membrane proteolipids displays a lower Vmax of the plasma membrane H(+)-ATPase activity.
Full Text
Duke Authors
Cited Authors
- Navarre, C; Catty, P; Leterme, S; Dietrich, F; Goffeau, A
Published Date
- August 19, 1994
Published In
Volume / Issue
- 269 / 33
Start / End Page
- 21262 - 21268
PubMed ID
- 8063750
International Standard Serial Number (ISSN)
- 0021-9258
Language
- eng
Conference Location
- United States