Identification of molybdopterin guanine dinucleotide in formate dehydrogenase from Methanobacterium formicicum.

Published

Journal Article

The pterin cofactor in formate dehydrogenase isolated from Methanobacterium formicium is identified as molybdopterin guanine dinucleotide. The pterin, stabilized as the alkylated, dicarboxamidomethyl derivative, is shown to have absorption and chromatographic properties identical to those of the previously characterized authentic compound. Treatment with nucleotide pyrophosphatase produced the expected degradation products GMP and carboxyamidomethyl molybdopterin. The molybdopterin guanine dinucleotide released from the enzyme by treatment with 95% dimethyl sulfoxide is shown to be functional in the in vitro reconstitution of the cofactor-deficient nitrate reductase in extracts of the Neurospora crassa nit-1 mutant.

Full Text

Duke Authors

Cited Authors

  • Johnson, JL; Bastian, NR; Schauer, NL; Ferry, JG; Rajagopalan, KV

Published Date

  • January 15, 1991

Published In

Volume / Issue

  • 61 / 2-3

Start / End Page

  • 213 - 216

PubMed ID

  • 2037231

Pubmed Central ID

  • 2037231

International Standard Serial Number (ISSN)

  • 0378-1097

Digital Object Identifier (DOI)

  • 10.1016/0378-1097(91)90554-n

Language

  • eng

Conference Location

  • England