Identification of molybdopterin guanine dinucleotide in formate dehydrogenase from Methanobacterium formicicum.
Journal Article (Journal Article)
The pterin cofactor in formate dehydrogenase isolated from Methanobacterium formicium is identified as molybdopterin guanine dinucleotide. The pterin, stabilized as the alkylated, dicarboxamidomethyl derivative, is shown to have absorption and chromatographic properties identical to those of the previously characterized authentic compound. Treatment with nucleotide pyrophosphatase produced the expected degradation products GMP and carboxyamidomethyl molybdopterin. The molybdopterin guanine dinucleotide released from the enzyme by treatment with 95% dimethyl sulfoxide is shown to be functional in the in vitro reconstitution of the cofactor-deficient nitrate reductase in extracts of the Neurospora crassa nit-1 mutant.
- Johnson, JL; Bastian, NR; Schauer, NL; Ferry, JG; Rajagopalan, KV
- January 15, 1991
Volume / Issue
- 61 / 2-3
Start / End Page
- 213 - 216
International Standard Serial Number (ISSN)
Digital Object Identifier (DOI)