The presence and distribution of reduced folates in Escherichia coli dihydrofolate reductase mutants.

Journal Article (Journal Article)

Escherichia coli DNA photolyase was overproduced and purified from each of two mutant E. coli strains lacking dihydrofolate reductase. The extent of over-production in the mutants was comparable to that seen in the wild type strain. Examination of the isolated photolyase from these strains revealed that the folate cofactor, 5,10-methenyltetrahydrofolate, was present in these proteins at a level of 60-80% compared to that purified from the wild type strain. Further examination of the dihydrofolate reductase-deficient strains revealed the presence of other tetrahydrofolate derivatives. These findings demonstrate that dihydrofolate reductase is not essential for the production of tetrahydrofolates in E. coli.

Full Text

Duke Authors

Cited Authors

  • Hamm-Alvarez, SF; Sancar, A; Rajagopalan, KV

Published Date

  • June 15, 1990

Published In

Volume / Issue

  • 265 / 17

Start / End Page

  • 9850 - 9856

PubMed ID

  • 2190985

International Standard Serial Number (ISSN)

  • 0021-9258


  • eng

Conference Location

  • United States