Selenite binding to carbon monoxide oxidase from Pseudomonas carboxydovorans. Selenium binds covalently to the protein and activates specifically the CO----methylene blue reaction.
Journal Article (Journal Article)
The CO----methylene blue and CO----dichlorophenol indophenol activities of carbon monoxide oxidase were specifically activated upon aerobic incubation with selenite, whereas the NADH----methylene blue activity was not altered. Fully active enzyme contained selenium, molybdenum, and flavin adenine dinucleotide in a 1:1:1 ratio. Selenium was covalently bound to the protein, probably between the sulfurs of half-cystine residues, and not a constituent of the molybdenum cofactor. The action of selenite was directed to the cytoplasmic species of carbon monoxide oxidase exclusively, whereas the CO----methylene blue activity of the membrane-bound enzyme remained unaffected.
Full Text
Duke Authors
Cited Authors
- Meyer, O; Rajagopalan, KV
Published Date
- May 10, 1984
Published In
Volume / Issue
- 259 / 9
Start / End Page
- 5612 - 5617
PubMed ID
- 6715362
International Standard Serial Number (ISSN)
- 0021-9258
Language
- eng
Conference Location
- United States