Selenite binding to carbon monoxide oxidase from Pseudomonas carboxydovorans. Selenium binds covalently to the protein and activates specifically the CO----methylene blue reaction.

Published

Journal Article

The CO----methylene blue and CO----dichlorophenol indophenol activities of carbon monoxide oxidase were specifically activated upon aerobic incubation with selenite, whereas the NADH----methylene blue activity was not altered. Fully active enzyme contained selenium, molybdenum, and flavin adenine dinucleotide in a 1:1:1 ratio. Selenium was covalently bound to the protein, probably between the sulfurs of half-cystine residues, and not a constituent of the molybdenum cofactor. The action of selenite was directed to the cytoplasmic species of carbon monoxide oxidase exclusively, whereas the CO----methylene blue activity of the membrane-bound enzyme remained unaffected.

Full Text

Duke Authors

Cited Authors

  • Meyer, O; Rajagopalan, KV

Published Date

  • May 10, 1984

Published In

Volume / Issue

  • 259 / 9

Start / End Page

  • 5612 - 5617

PubMed ID

  • 6715362

Pubmed Central ID

  • 6715362

International Standard Serial Number (ISSN)

  • 0021-9258

Language

  • eng

Conference Location

  • United States