Immunohistochemical demonstration of glycogen phosphorylase in rat brain slices.

Published

Journal Article

Paraffin-embedded sections from paraformaldehyde-fixed rat brain were stained immunocytochemically for glycogen phosphorylase brain isozyme BB, using a monoclonal mouse antibody and the biotin-strept-avidin method, with either horseradish peroxidase or beta-galactosidase as marker enzymes. Two cell types showed strong glycogen phosphorylase-immunoreactivity: Astrocytes and ependymal cells. Most intensive staining was observed in the cerebellar cortex, the neocortex and the hippocampus. Astrocytes in the cerebellar white matter stained positively. The choroid plexus cells stained poorly or not at all. Neurons throughout the brain were negative, as well as oligodendrocytes and bundles of myelinated nerve fibers. These data are consistent with the immunocytochemical localization of glycogen phosphorylase in astroglia-rich primary cultures derived from rat brain.

Full Text

Cited Authors

  • Pfeiffer, B; Elmer, K; Roggendorf, W; Reinhart, PH; Hamprecht, B

Published Date

  • January 1990

Published In

Volume / Issue

  • 94 / 1

Start / End Page

  • 73 - 80

PubMed ID

  • 2351562

Pubmed Central ID

  • 2351562

International Standard Serial Number (ISSN)

  • 0301-5564

Digital Object Identifier (DOI)

  • 10.1007/bf00266792

Language

  • eng