Reinterpretation of GCN4-p1 folding kinetics: partial helix formation precedes dimerization in coiled coil folding.
Publication
, Journal Article
Myers, JK; Oas, TG
Published in: J Mol Biol
June 4, 1999
The folding of coiled coil peptides has traditionally been interpreted in terms of native dimer and unfolded monomers. Calculations using AGADIR and experimental studies of fragments suggest that the monomers of the coiled coil peptide, GCN4-p1, contain significant residual helical structure. A simple model based on diffusion-collision theory predicts not only the measured folding rate within an order of magnitude, but also predicts remarkably well the effect of alanine to glyXcine mutations. We suggest that intrinsic helix stability is a major determinant of the folding rate of the GCN4 coiled coil.
Duke Scholars
Published In
J Mol Biol
DOI
ISSN
0022-2836
Publication Date
June 4, 1999
Volume
289
Issue
2
Start / End Page
205 / 209
Location
Netherlands
Related Subject Headings
- Thermodynamics
- Saccharomyces cerevisiae Proteins
- Recombinant Proteins
- Protein Structure, Secondary
- Protein Kinases
- Protein Folding
- Mutagenesis, Site-Directed
- Kinetics
- Glycine
- Fungal Proteins
Citation
APA
Chicago
ICMJE
MLA
NLM
Myers, J. K., & Oas, T. G. (1999). Reinterpretation of GCN4-p1 folding kinetics: partial helix formation precedes dimerization in coiled coil folding. J Mol Biol, 289(2), 205–209. https://doi.org/10.1006/jmbi.1999.2747
Myers, J. K., and T. G. Oas. “Reinterpretation of GCN4-p1 folding kinetics: partial helix formation precedes dimerization in coiled coil folding.” J Mol Biol 289, no. 2 (June 4, 1999): 205–9. https://doi.org/10.1006/jmbi.1999.2747.
Myers JK, Oas TG. Reinterpretation of GCN4-p1 folding kinetics: partial helix formation precedes dimerization in coiled coil folding. J Mol Biol. 1999 Jun 4;289(2):205–9.
Myers, J. K., and T. G. Oas. “Reinterpretation of GCN4-p1 folding kinetics: partial helix formation precedes dimerization in coiled coil folding.” J Mol Biol, vol. 289, no. 2, June 1999, pp. 205–09. Pubmed, doi:10.1006/jmbi.1999.2747.
Myers JK, Oas TG. Reinterpretation of GCN4-p1 folding kinetics: partial helix formation precedes dimerization in coiled coil folding. J Mol Biol. 1999 Jun 4;289(2):205–209.
Published In
J Mol Biol
DOI
ISSN
0022-2836
Publication Date
June 4, 1999
Volume
289
Issue
2
Start / End Page
205 / 209
Location
Netherlands
Related Subject Headings
- Thermodynamics
- Saccharomyces cerevisiae Proteins
- Recombinant Proteins
- Protein Structure, Secondary
- Protein Kinases
- Protein Folding
- Mutagenesis, Site-Directed
- Kinetics
- Glycine
- Fungal Proteins