Transgenic mice expressing a constitutively active retinoic acid receptor in the lens exhibit ocular defects.

Journal Article (Journal Article)

Retinoic acid receptors (RARs) modulate gene expression following association with retinoic acid (RA). In transient transfection, an RAR alpha-beta-galactosidase fusion protein (RAR-LacZ) was able to transactivate expression in the absence of RA. When expressed in the ocular lens of transgenic mice, this constitutively active RAR-LacZ fusion gene resulted in founder and progeny animals that exhibited cataracts and microphthalmia, both being characteristics of retinoid-induced teratogenesis. The transgenic phenotypes indicate that retinoid teratogenesis can be mimicked by expression of a constitutively active RAR-LacZ fusion protein in retinoid-sensitive tissues.

Full Text

Duke Authors

Cited Authors

  • Balkan, W; Klintworth, GK; Bock, CB; Linney, E

Published Date

  • June 1992

Published In

Volume / Issue

  • 151 / 2

Start / End Page

  • 622 - 625

PubMed ID

  • 1318236

International Standard Serial Number (ISSN)

  • 0012-1606

Digital Object Identifier (DOI)

  • 10.1016/0012-1606(92)90200-z


  • eng

Conference Location

  • United States