Transgenic mice expressing a constitutively active retinoic acid receptor in the lens exhibit ocular defects.
Journal Article (Journal Article)
Retinoic acid receptors (RARs) modulate gene expression following association with retinoic acid (RA). In transient transfection, an RAR alpha-beta-galactosidase fusion protein (RAR-LacZ) was able to transactivate expression in the absence of RA. When expressed in the ocular lens of transgenic mice, this constitutively active RAR-LacZ fusion gene resulted in founder and progeny animals that exhibited cataracts and microphthalmia, both being characteristics of retinoid-induced teratogenesis. The transgenic phenotypes indicate that retinoid teratogenesis can be mimicked by expression of a constitutively active RAR-LacZ fusion protein in retinoid-sensitive tissues.
- Balkan, W; Klintworth, GK; Bock, CB; Linney, E
- June 1992
Volume / Issue
- 151 / 2
Start / End Page
- 622 - 625
International Standard Serial Number (ISSN)
Digital Object Identifier (DOI)
- United States