Intracorneal bovine albumin: an immunologic model of corneal angiogenesis.


Journal Article

BACKGROUND: We characterized the neovascularization that follows the intracorneal injection of bovine albumin (BA) in rabbits as a model of corneal angiogenesis. METHODS: New Zealand white rabbits received intracorneal injections of phosphate-buffered saline with and without various amounts of BA. The rabbits were co-sensitized or pre-sensitized by intramuscular BA or were not sensitized. The corneal vascular response was quantified by ranking photographs taken periodically after the injection. RESULTS: In pre-sensitized animals, blood vessels were apparent within 4 days and reached maximum intensity 14 days after the intracorneal injection. Corneas also vascularized in non-sensitized rabbits, but a larger dose (> 0.2 mg BA) was required than in pre-sensitized animals (> 0.02 mg BA). Vascularization began later in non-sensitized animals and was less extensive than in pre-sensitized animals. CONCLUSION: The intracorneal injection of BA is a reproducible model of corneal angiogenesis in rabbits and should allow the involved immunological mechanisms to be elucidated.

Full Text

Cited Authors

  • Damms, T; Ross, JR; Duplessie, MD; Klintworth, GK

Published Date

  • October 1997

Published In

Volume / Issue

  • 235 / 10

Start / End Page

  • 662 - 666

PubMed ID

  • 9349952

Pubmed Central ID

  • 9349952

International Standard Serial Number (ISSN)

  • 0721-832X

Digital Object Identifier (DOI)

  • 10.1007/bf00946944


  • eng

Conference Location

  • Germany