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Fluorescence-based isolation of bacterial genes expressed within host cells.

Publication ,  Journal Article
Valdivia, RH; Falkow, S
Published in: Science
September 26, 1997

A selection strategy was devised to identify bacterial genes preferentially expressed when a bacterium associates with its host cell. Fourteen Salmonella typhimurium genes, which were under the control of at least four independent regulatory circuits, were identified to be selectively induced in host macrophages. Four genes encode virulence factors, including a component of a type III secretory apparatus. This selection methodology should be generally applicable to the identification of genes from pathogenic organisms that are induced upon association with host cells or tissues.

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Published In

Science

DOI

ISSN

0036-8075

Publication Date

September 26, 1997

Volume

277

Issue

5334

Start / End Page

2007 / 2011

Location

United States

Related Subject Headings

  • Virulence
  • Transcription Factors
  • Spleen
  • Salmonella typhimurium
  • Salmonella Infections, Animal
  • Recombinant Fusion Proteins
  • Promoter Regions, Genetic
  • Open Reading Frames
  • Molecular Sequence Data
  • Microscopy, Fluorescence
 

Citation

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Valdivia, R. H., & Falkow, S. (1997). Fluorescence-based isolation of bacterial genes expressed within host cells. Science, 277(5334), 2007–2011. https://doi.org/10.1126/science.277.5334.2007
Valdivia, R. H., and S. Falkow. “Fluorescence-based isolation of bacterial genes expressed within host cells.Science 277, no. 5334 (September 26, 1997): 2007–11. https://doi.org/10.1126/science.277.5334.2007.
Valdivia RH, Falkow S. Fluorescence-based isolation of bacterial genes expressed within host cells. Science. 1997 Sep 26;277(5334):2007–11.
Valdivia, R. H., and S. Falkow. “Fluorescence-based isolation of bacterial genes expressed within host cells.Science, vol. 277, no. 5334, Sept. 1997, pp. 2007–11. Pubmed, doi:10.1126/science.277.5334.2007.
Valdivia RH, Falkow S. Fluorescence-based isolation of bacterial genes expressed within host cells. Science. 1997 Sep 26;277(5334):2007–2011.
Journal cover image

Published In

Science

DOI

ISSN

0036-8075

Publication Date

September 26, 1997

Volume

277

Issue

5334

Start / End Page

2007 / 2011

Location

United States

Related Subject Headings

  • Virulence
  • Transcription Factors
  • Spleen
  • Salmonella typhimurium
  • Salmonella Infections, Animal
  • Recombinant Fusion Proteins
  • Promoter Regions, Genetic
  • Open Reading Frames
  • Molecular Sequence Data
  • Microscopy, Fluorescence