Fluorescence-based isolation of bacterial genes expressed within host cells.
Publication
, Journal Article
Valdivia, RH; Falkow, S
Published in: Science
September 26, 1997
A selection strategy was devised to identify bacterial genes preferentially expressed when a bacterium associates with its host cell. Fourteen Salmonella typhimurium genes, which were under the control of at least four independent regulatory circuits, were identified to be selectively induced in host macrophages. Four genes encode virulence factors, including a component of a type III secretory apparatus. This selection methodology should be generally applicable to the identification of genes from pathogenic organisms that are induced upon association with host cells or tissues.
Duke Scholars
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Published In
Science
DOI
ISSN
0036-8075
Publication Date
September 26, 1997
Volume
277
Issue
5334
Start / End Page
2007 / 2011
Location
United States
Related Subject Headings
- Virulence
- Transcription Factors
- Spleen
- Salmonella typhimurium
- Salmonella Infections, Animal
- Recombinant Fusion Proteins
- Promoter Regions, Genetic
- Open Reading Frames
- Molecular Sequence Data
- Microscopy, Fluorescence
Citation
APA
Chicago
ICMJE
MLA
NLM
Valdivia, R. H., & Falkow, S. (1997). Fluorescence-based isolation of bacterial genes expressed within host cells. Science, 277(5334), 2007–2011. https://doi.org/10.1126/science.277.5334.2007
Valdivia, R. H., and S. Falkow. “Fluorescence-based isolation of bacterial genes expressed within host cells.” Science 277, no. 5334 (September 26, 1997): 2007–11. https://doi.org/10.1126/science.277.5334.2007.
Valdivia RH, Falkow S. Fluorescence-based isolation of bacterial genes expressed within host cells. Science. 1997 Sep 26;277(5334):2007–11.
Valdivia, R. H., and S. Falkow. “Fluorescence-based isolation of bacterial genes expressed within host cells.” Science, vol. 277, no. 5334, Sept. 1997, pp. 2007–11. Pubmed, doi:10.1126/science.277.5334.2007.
Valdivia RH, Falkow S. Fluorescence-based isolation of bacterial genes expressed within host cells. Science. 1997 Sep 26;277(5334):2007–2011.
Published In
Science
DOI
ISSN
0036-8075
Publication Date
September 26, 1997
Volume
277
Issue
5334
Start / End Page
2007 / 2011
Location
United States
Related Subject Headings
- Virulence
- Transcription Factors
- Spleen
- Salmonella typhimurium
- Salmonella Infections, Animal
- Recombinant Fusion Proteins
- Promoter Regions, Genetic
- Open Reading Frames
- Molecular Sequence Data
- Microscopy, Fluorescence