Fluorescence-based isolation of bacterial genes expressed within host cells.


Journal Article

A selection strategy was devised to identify bacterial genes preferentially expressed when a bacterium associates with its host cell. Fourteen Salmonella typhimurium genes, which were under the control of at least four independent regulatory circuits, were identified to be selectively induced in host macrophages. Four genes encode virulence factors, including a component of a type III secretory apparatus. This selection methodology should be generally applicable to the identification of genes from pathogenic organisms that are induced upon association with host cells or tissues.

Full Text

Duke Authors

Cited Authors

  • Valdivia, RH; Falkow, S

Published Date

  • September 26, 1997

Published In

Volume / Issue

  • 277 / 5334

Start / End Page

  • 2007 - 2011

PubMed ID

  • 9302299

Pubmed Central ID

  • 9302299

International Standard Serial Number (ISSN)

  • 0036-8075

Digital Object Identifier (DOI)

  • 10.1126/science.277.5334.2007


  • eng

Conference Location

  • United States