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Applications for green fluorescent protein (GFP) in the study of host-pathogen interactions.

Publication ,  Journal Article
Valdivia, RH; Hromockyj, AE; Monack, D; Ramakrishnan, L; Falkow, S
Published in: Gene
1996

The green fluorescent protein (GFP) from Aequorea victoria is a novel fluorescent marker that has potential use in the study of bacterial pathogenicity. To explore some of the potential applications of GFP to the study of host-parasite interactions, we constructed two GFP expression vectors suitable for different facultative intracellular bacterial pathogens. The first expression vector was tested in the enteric pathogens, Salmonella typhimurium and Yersinia pseudotuberculosis, and the second vector tested in Mycobacterium marinum (Mm). Both expression vectors were found to be stable and to direct high levels of GFP synthesis. Standard epifluorescence microscopy was used to detect all three bacterial pathogenic species during the early and late stages of infection of live mammalian cells. Mm expressing gfp was also visualized in infected animal tissues. gfp expression did not adversely affect bacterial survival, nor did it compromise entry into mammalian cells or their survival within macrophages. In addition, all three gfp-expressing bacterial pathogens could be detected and sorted in a flow cytometer, either alone or in association with epithelial cells or macrophages. Therefore, GFP not only provides a convenient tool to image pathogenic bacteria, but allows the quantitative measurement of bacterial association with mammalian cells.

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Published In

Gene

DOI

ISSN

0378-1119

Publication Date

1996

Volume

173

Issue

1 Spec No

Start / End Page

47 / 52

Location

Netherlands

Related Subject Headings

  • Yersinia pseudotuberculosis
  • Tumor Cells, Cultured
  • Spleen
  • Scyphozoa
  • Salmonella typhimurium
  • Rana pipiens
  • Mycobacterium
  • Molecular Sequence Data
  • Mice
  • Macrophages
 

Citation

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ICMJE
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Valdivia, R. H., Hromockyj, A. E., Monack, D., Ramakrishnan, L., & Falkow, S. (1996). Applications for green fluorescent protein (GFP) in the study of host-pathogen interactions. Gene, 173(1 Spec No), 47–52. https://doi.org/10.1016/0378-1119(95)00706-7
Valdivia, R. H., A. E. Hromockyj, D. Monack, L. Ramakrishnan, and S. Falkow. “Applications for green fluorescent protein (GFP) in the study of host-pathogen interactions.Gene 173, no. 1 Spec No (1996): 47–52. https://doi.org/10.1016/0378-1119(95)00706-7.
Valdivia RH, Hromockyj AE, Monack D, Ramakrishnan L, Falkow S. Applications for green fluorescent protein (GFP) in the study of host-pathogen interactions. Gene. 1996;173(1 Spec No):47–52.
Valdivia, R. H., et al. “Applications for green fluorescent protein (GFP) in the study of host-pathogen interactions.Gene, vol. 173, no. 1 Spec No, 1996, pp. 47–52. Pubmed, doi:10.1016/0378-1119(95)00706-7.
Valdivia RH, Hromockyj AE, Monack D, Ramakrishnan L, Falkow S. Applications for green fluorescent protein (GFP) in the study of host-pathogen interactions. Gene. 1996;173(1 Spec No):47–52.
Journal cover image

Published In

Gene

DOI

ISSN

0378-1119

Publication Date

1996

Volume

173

Issue

1 Spec No

Start / End Page

47 / 52

Location

Netherlands

Related Subject Headings

  • Yersinia pseudotuberculosis
  • Tumor Cells, Cultured
  • Spleen
  • Scyphozoa
  • Salmonella typhimurium
  • Rana pipiens
  • Mycobacterium
  • Molecular Sequence Data
  • Mice
  • Macrophages