Detection of trisomy 12 by fluorescence in situ hybridization on archival cytopathologic material in chronic lymphocytic leukemia/small lymphocytic lymphoma.

Published

Journal Article

OBJECTIVE: To evaluate fluorescence in situ hybridization (FISH) for the detection of trisomy 12 in archival cytologic specimens of chronic lymphocytic leukemia/small lymphocytic lymphoma. STUDY DESIGN: The cytopathology database was searched for all cases of chronic lymphocytic leukemia/small lymphocytic lymphoma. Six cases of chronic lymphocytic leukemia/small lymphocytic lymphoma obtained by fine needle aspiration and one case of small lymphocytic lymphoma with plasmacytoid features were analyzed for trisomy 12 by FISH. These cases had been archived between 1 week to 16 months prior to analysis. RESULTS: We detected trisomy 12 in four of the six cases of small lymphocytic lymphoma/chronic lymphocytic leukemia. The case of small lymphocytic lymphoma with plasmacytoid features was negative for trisomy 12. CONCLUSION: Detection of trisomy 12 by FISH can be effectively performed on routinely prepared, stained and coverslipped archival cytologic material.

Full Text

Duke Authors

Cited Authors

  • Liu, K; Dodd, LG; Powers, JA; Bigner, SH

Published Date

  • May 2000

Published In

Volume / Issue

  • 44 / 3

Start / End Page

  • 368 - 374

PubMed ID

  • 10833993

Pubmed Central ID

  • 10833993

International Standard Serial Number (ISSN)

  • 0001-5547

Digital Object Identifier (DOI)

  • 10.1159/000328480

Language

  • eng

Conference Location

  • Switzerland