Tn5-mediated bleomycin resistance in Escherichia coli requires the expression of host genes.

Journal Article (Journal Article)

The transposon Tn5 expresses a gene, ble, whose product increases the viability of Escherichia coli and also confers resistance to the DNA-cleaving antibiotic bleomycin and the DNA-alkylating agent ethylmethanesulphonate. We find that the Ble protein induces expression of an alkylation inducible gene, aidC, and that both the AidC gene product and DNA polymerase I are required for Ble to confer bleomycin resistance. These findings support models in which Ble enhances DNA repair and suggest that Tn5 confers a fitness advantage to the host bacterium by increasing the repair of spontaneous DNA lesions. Such co-operation between a transposon and its host suggests that Tn5 is a symbiotic rather than a selfish DNA element.

Full Text

Duke Authors

Cited Authors

  • Blot, M; Heitman, J; Arber, W

Published Date

  • June 1993

Published In

Volume / Issue

  • 8 / 6

Start / End Page

  • 1017 - 1024

PubMed ID

  • 7689686

International Standard Serial Number (ISSN)

  • 0950-382X

Digital Object Identifier (DOI)

  • 10.1111/j.1365-2958.1993.tb01646.x


  • eng

Conference Location

  • England