How the EcoRI endonuclease recognizes and cleaves DNA.

Published

Journal Article (Review)

One popular recombinant DNA tool is the EcoRI endonuclease, which cleaves DNA at GAATTC sites and serves as a paradigm for sequence specific DNA-enzyme interactions. The recently revised X-ray crystal structure of an EcoRI-DNA complex reveals EcoRI employs novel DNA recognition motifs, a four alpha-helix bundle and two extended chains, which project into the major groove to contact substrate purines and pyrimidines. Interestingly, pyrimidine contacts had been predicted based on genetic and biochemical studies. Current work focuses on the EcoRI active site structure, enzyme and substrate conformational changes during catalysis, and host-restriction system interactions.

Full Text

Duke Authors

Cited Authors

  • Heitman, J

Published Date

  • July 1992

Published In

Volume / Issue

  • 14 / 7

Start / End Page

  • 445 - 454

PubMed ID

  • 1445286

Pubmed Central ID

  • 1445286

International Standard Serial Number (ISSN)

  • 0265-9247

Digital Object Identifier (DOI)

  • 10.1002/bies.950140704

Language

  • eng

Conference Location

  • United States