How the EcoRI endonuclease recognizes and cleaves DNA.
Publication
, Journal Article
Heitman, J
Published in: Bioessays
July 1992
One popular recombinant DNA tool is the EcoRI endonuclease, which cleaves DNA at GAATTC sites and serves as a paradigm for sequence specific DNA-enzyme interactions. The recently revised X-ray crystal structure of an EcoRI-DNA complex reveals EcoRI employs novel DNA recognition motifs, a four alpha-helix bundle and two extended chains, which project into the major groove to contact substrate purines and pyrimidines. Interestingly, pyrimidine contacts had been predicted based on genetic and biochemical studies. Current work focuses on the EcoRI active site structure, enzyme and substrate conformational changes during catalysis, and host-restriction system interactions.
Duke Scholars
Published In
Bioessays
DOI
ISSN
0265-9247
Publication Date
July 1992
Volume
14
Issue
7
Start / End Page
445 / 454
Location
United States
Related Subject Headings
- X-Ray Diffraction
- Substrate Specificity
- Protein Conformation
- Protein Binding
- Nucleic Acid Conformation
- Molecular Sequence Data
- Models, Molecular
- Escherichia coli
- Developmental Biology
- Deoxyribonuclease EcoRI
Citation
APA
Chicago
ICMJE
MLA
NLM
Heitman, J. (1992). How the EcoRI endonuclease recognizes and cleaves DNA. Bioessays, 14(7), 445–454. https://doi.org/10.1002/bies.950140704
Heitman, J. “How the EcoRI endonuclease recognizes and cleaves DNA.” Bioessays 14, no. 7 (July 1992): 445–54. https://doi.org/10.1002/bies.950140704.
Heitman J. How the EcoRI endonuclease recognizes and cleaves DNA. Bioessays. 1992 Jul;14(7):445–54.
Heitman, J. “How the EcoRI endonuclease recognizes and cleaves DNA.” Bioessays, vol. 14, no. 7, July 1992, pp. 445–54. Pubmed, doi:10.1002/bies.950140704.
Heitman J. How the EcoRI endonuclease recognizes and cleaves DNA. Bioessays. 1992 Jul;14(7):445–454.
Published In
Bioessays
DOI
ISSN
0265-9247
Publication Date
July 1992
Volume
14
Issue
7
Start / End Page
445 / 454
Location
United States
Related Subject Headings
- X-Ray Diffraction
- Substrate Specificity
- Protein Conformation
- Protein Binding
- Nucleic Acid Conformation
- Molecular Sequence Data
- Models, Molecular
- Escherichia coli
- Developmental Biology
- Deoxyribonuclease EcoRI