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Repair of the Escherichia coli chromosome after in vivo scission by the EcoRI endonuclease.

Publication ,  Journal Article
Heitman, J; Zinder, ND; Model, P
Published in: Proc Natl Acad Sci U S A
April 1989
Published version (DOI) Link to item

We prepared a set of temperature-sensitive mutants of the EcoRI endonuclease. Under semipermissive conditions, Escherichia coli strains bearing these alleles form poorly growing colonies in which intracellular substrates are cleaved at EcoRI sites and the SOS DNA repair response is induced. Strains defective in SOS induction (lexA3 mutant) or SOS induction and recombination (recA56 and recB21 mutants) are not more sensitive to this in vivo DNA scission, whereas strains deficient in DNA ligase (lig4 and lig ts7 mutants) are extremely sensitive. We conclude that although DNA scission induces the SOS response, neither this induction nor recombination are required for repair. DNA ligase is necessary and may be sufficient to repair EcoRI-mediated DNA breaks in the E. coli chromosome.

Duke Scholars

Joseph Heitman
Author Joseph Heitman Molecular Genetics and Microbiology

Published In

Proc Natl Acad Sci U S A

DOI

10.1073/pnas.86.7.2281

ISSN

0027-8424

Publication Date

April 1989

Volume

86

Issue

7

Start / End Page

2281 / 2285

Location

United States

Related Subject Headings

  • Restriction Mapping
  • Plasmids
  • Mutation
  • Kinetics
  • Genotype
  • Escherichia coli
  • Deoxyribonuclease EcoRI
  • DNA Repair
  • DNA Damage
  • Chromosomes, Bacterial
 

Citation

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Heitman, J., Zinder, N. D., & Model, P. (1989). Repair of the Escherichia coli chromosome after in vivo scission by the EcoRI endonuclease. Proc Natl Acad Sci U S A, 86(7), 2281–2285. https://doi.org/10.1073/pnas.86.7.2281
Heitman, J., N. D. Zinder, and P. Model. “Repair of the Escherichia coli chromosome after in vivo scission by the EcoRI endonuclease.” Proc Natl Acad Sci U S A 86, no. 7 (April 1989): 2281–85. https://doi.org/10.1073/pnas.86.7.2281.
Heitman J, Zinder ND, Model P. Repair of the Escherichia coli chromosome after in vivo scission by the EcoRI endonuclease. Proc Natl Acad Sci U S A. 1989 Apr;86(7):2281–5.
Heitman, J., et al. “Repair of the Escherichia coli chromosome after in vivo scission by the EcoRI endonuclease.” Proc Natl Acad Sci U S A, vol. 86, no. 7, Apr. 1989, pp. 2281–85. Pubmed, doi:10.1073/pnas.86.7.2281.
Heitman J, Zinder ND, Model P. Repair of the Escherichia coli chromosome after in vivo scission by the EcoRI endonuclease. Proc Natl Acad Sci U S A. 1989 Apr;86(7):2281–2285.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

10.1073/pnas.86.7.2281

ISSN

0027-8424

Publication Date

April 1989

Volume

86

Issue

7

Start / End Page

2281 / 2285

Location

United States

Related Subject Headings

  • Restriction Mapping
  • Plasmids
  • Mutation
  • Kinetics
  • Genotype
  • Escherichia coli
  • Deoxyribonuclease EcoRI
  • DNA Repair
  • DNA Damage
  • Chromosomes, Bacterial