Repair of the Escherichia coli chromosome after in vivo scission by the EcoRI endonuclease.
Journal Article (Journal Article)
We prepared a set of temperature-sensitive mutants of the EcoRI endonuclease. Under semipermissive conditions, Escherichia coli strains bearing these alleles form poorly growing colonies in which intracellular substrates are cleaved at EcoRI sites and the SOS DNA repair response is induced. Strains defective in SOS induction (lexA3 mutant) or SOS induction and recombination (recA56 and recB21 mutants) are not more sensitive to this in vivo DNA scission, whereas strains deficient in DNA ligase (lig4 and lig ts7 mutants) are extremely sensitive. We conclude that although DNA scission induces the SOS response, neither this induction nor recombination are required for repair. DNA ligase is necessary and may be sufficient to repair EcoRI-mediated DNA breaks in the E. coli chromosome.
Full Text
Duke Authors
Cited Authors
- Heitman, J; Zinder, ND; Model, P
Published Date
- April 1989
Published In
Volume / Issue
- 86 / 7
Start / End Page
- 2281 - 2285
PubMed ID
- 2648397
Pubmed Central ID
- PMC286896
International Standard Serial Number (ISSN)
- 0027-8424
Digital Object Identifier (DOI)
- 10.1073/pnas.86.7.2281
Language
- eng
Conference Location
- United States