Repair of the Escherichia coli chromosome after in vivo scission by the EcoRI endonuclease.

Published

Journal Article

We prepared a set of temperature-sensitive mutants of the EcoRI endonuclease. Under semipermissive conditions, Escherichia coli strains bearing these alleles form poorly growing colonies in which intracellular substrates are cleaved at EcoRI sites and the SOS DNA repair response is induced. Strains defective in SOS induction (lexA3 mutant) or SOS induction and recombination (recA56 and recB21 mutants) are not more sensitive to this in vivo DNA scission, whereas strains deficient in DNA ligase (lig4 and lig ts7 mutants) are extremely sensitive. We conclude that although DNA scission induces the SOS response, neither this induction nor recombination are required for repair. DNA ligase is necessary and may be sufficient to repair EcoRI-mediated DNA breaks in the E. coli chromosome.

Full Text

Duke Authors

Cited Authors

  • Heitman, J; Zinder, ND; Model, P

Published Date

  • April 1989

Published In

Volume / Issue

  • 86 / 7

Start / End Page

  • 2281 - 2285

PubMed ID

  • 2648397

Pubmed Central ID

  • 2648397

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.86.7.2281

Language

  • eng

Conference Location

  • United States