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Protein kinase A operates a molecular switch that governs yeast pseudohyphal differentiation.

Publication ,  Journal Article
Pan, X; Heitman, J
Published in: Mol Cell Biol
June 2002

The yeast Saccharomyces cerevisiae undergoes a dimorphic filamentous transition in response to nutrient cues that is affected by both mitogen-activated protein kinase and cyclic AMP-protein kinase A signaling cascades. Here two transcriptional regulators, Flo8 and Sfl1, are shown to be the direct molecular targets of protein kinase A. Flo8 and Sfl1 antagonistically control expression of the cell adhesin Flo11 via a common promoter element. Phosphorylation by the protein kinase A catalytic subunit Tpk2 promotes Flo8 binding and activation of the Flo11 promoter and relieves repression by prohibiting dimerization and DNA binding by Sfl1. Our studies illustrate in molecular detail how protein kinase A combinatorially effects a key developmental switch. Similar mechanisms may operate in pathogenic fungi and more complex multicellular eukaryotic organisms.

Duke Scholars

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Published In

Mol Cell Biol

DOI

ISSN

0270-7306

Publication Date

June 2002

Volume

22

Issue

12

Start / End Page

3981 / 3993

Location

United States

Related Subject Headings

  • Transcription Factors
  • Trans-Activators
  • Serine Endopeptidases
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Protein Kinases
  • Promoter Regions, Genetic
  • Phosphorylation
  • Nuclear Proteins
  • Mutation
 

Citation

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Pan, X., & Heitman, J. (2002). Protein kinase A operates a molecular switch that governs yeast pseudohyphal differentiation. Mol Cell Biol, 22(12), 3981–3993. https://doi.org/10.1128/MCB.22.12.3981-3993.2002
Pan, Xuewen, and Joseph Heitman. “Protein kinase A operates a molecular switch that governs yeast pseudohyphal differentiation.Mol Cell Biol 22, no. 12 (June 2002): 3981–93. https://doi.org/10.1128/MCB.22.12.3981-3993.2002.
Pan, Xuewen, and Joseph Heitman. “Protein kinase A operates a molecular switch that governs yeast pseudohyphal differentiation.Mol Cell Biol, vol. 22, no. 12, June 2002, pp. 3981–93. Pubmed, doi:10.1128/MCB.22.12.3981-3993.2002.
Pan X, Heitman J. Protein kinase A operates a molecular switch that governs yeast pseudohyphal differentiation. Mol Cell Biol. 2002 Jun;22(12):3981–3993.

Published In

Mol Cell Biol

DOI

ISSN

0270-7306

Publication Date

June 2002

Volume

22

Issue

12

Start / End Page

3981 / 3993

Location

United States

Related Subject Headings

  • Transcription Factors
  • Trans-Activators
  • Serine Endopeptidases
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Protein Kinases
  • Promoter Regions, Genetic
  • Phosphorylation
  • Nuclear Proteins
  • Mutation