Killing of Caenorhabditis elegans by Cryptococcus neoformans as a model of yeast pathogenesis.

Journal Article (Journal Article)

We found that the well-studied nematode Caenorhabditis elegans can use various yeasts, including Cryptococcus laurentii and Cryptococcus kuetzingii, as a sole source of food, producing similar brood sizes compared with growth on its usual laboratory food source Escherichia coli OP50. C. elegans grown on these yeasts had a life span similar to (C. laurentii) or longer than (C. kuetzingii) those fed on E. coli. However, the human pathogenic yeast Cryptococcus neoformans killed C. elegans, and the C. neoformans polysaccharide capsule as well as several C. neoformans genes previously shown to be involved in mammalian virulence were also shown to play a role in C. elegans killing. These included genes associated with signal transduction pathways (GPA1, PKA1, PKR1, and RAS1), laccase production (LAC1), and the alpha mating type. C. neoformans adenine auxotrophs, which are less virulent in mammals, were also less virulent in C. elegans. These results support the model that mammalian pathogenesis of C. neoformans may be a consequence of adaptations that have evolved during the interaction of C. neoformans with environmental predators such as free-living nematodes and amoebae and suggest that C. elegans can be used as a simple model host in which C. neoformans pathogenesis can be readily studied.

Full Text

Duke Authors

Cited Authors

  • Mylonakis, E; Ausubel, FM; Perfect, JR; Heitman, J; Calderwood, SB

Published Date

  • November 26, 2002

Published In

Volume / Issue

  • 99 / 24

Start / End Page

  • 15675 - 15680

PubMed ID

  • 12438649

Pubmed Central ID

  • PMC137775

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.232568599


  • eng

Conference Location

  • United States