Skip to main content

Site-specific methylases induce the SOS DNA repair response in Escherichia coli.

Publication ,  Journal Article
Heitman, J; Model, P
Published in: J Bacteriol
July 1987

Expression of the site-specific adenine methylase HhaII (GmeANTC, where me is methyl) or PstI (CTGCmeAG) induced the SOS DNA repair response in Escherichia coli. In contrast, expression of methylases indigenous to E. coli either did not induce SOS (EcoRI [GAmeATTC] or induced SOS to a lesser extent (dam [GmeATC]). Recognition of adenine-methylated DNA required the product of a previously undescribed gene, which we named mrr (methylated adenine recognition and restriction). We suggest that mrr encodes an endonuclease that cleaves DNA containing N6-methyladenine and that DNA double-strand breaks induce the SOS response. Cytosine methylases foreign to E. coli (MspI [meCCGG], HaeIII [GGmeCC], BamHI [GGATmeCC], HhaI [GmeCGC], BsuRI [GGmeCC], and M.Spr) also induced SOS, whereas one indigenous to E. coli (EcoRII [CmeCA/TGG]) did not. SOS induction by cytosine methylation required the rglB locus, which encodes an endonuclease that cleaves DNA containing 5-hydroxymethyl- or 5-methylcytosine (E. A. Raleigh and G. Wilson, Proc. Natl. Acad. Sci. USA 83:9070-9074, 1986).

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

J Bacteriol

DOI

ISSN

0021-9193

Publication Date

July 1987

Volume

169

Issue

7

Start / End Page

3243 / 3250

Location

United States

Related Subject Headings

  • Site-Specific DNA-Methyltransferase (Adenine-Specific)
  • SOS Response, Genetics
  • Promoter Regions, Genetic
  • Mutation
  • Microbiology
  • Methyltransferases
  • Lysogeny
  • Escherichia coli
  • DNA Transposable Elements
  • DNA Repair
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Heitman, J., & Model, P. (1987). Site-specific methylases induce the SOS DNA repair response in Escherichia coli. J Bacteriol, 169(7), 3243–3250. https://doi.org/10.1128/jb.169.7.3243-3250.1987
Heitman, J., and P. Model. “Site-specific methylases induce the SOS DNA repair response in Escherichia coli.J Bacteriol 169, no. 7 (July 1987): 3243–50. https://doi.org/10.1128/jb.169.7.3243-3250.1987.
Heitman J, Model P. Site-specific methylases induce the SOS DNA repair response in Escherichia coli. J Bacteriol. 1987 Jul;169(7):3243–50.
Heitman, J., and P. Model. “Site-specific methylases induce the SOS DNA repair response in Escherichia coli.J Bacteriol, vol. 169, no. 7, July 1987, pp. 3243–50. Pubmed, doi:10.1128/jb.169.7.3243-3250.1987.
Heitman J, Model P. Site-specific methylases induce the SOS DNA repair response in Escherichia coli. J Bacteriol. 1987 Jul;169(7):3243–3250.

Published In

J Bacteriol

DOI

ISSN

0021-9193

Publication Date

July 1987

Volume

169

Issue

7

Start / End Page

3243 / 3250

Location

United States

Related Subject Headings

  • Site-Specific DNA-Methyltransferase (Adenine-Specific)
  • SOS Response, Genetics
  • Promoter Regions, Genetic
  • Mutation
  • Microbiology
  • Methyltransferases
  • Lysogeny
  • Escherichia coli
  • DNA Transposable Elements
  • DNA Repair