SOS induction as an in vivo assay of enzyme-DNA interactions.

Journal Article (Journal Article)

We have constructed strains which are convenient and sensitive indicators of DNA damage and describe their use. These strains utilize an SOS::lac Z fusion constructed by Kenyon and Walker [Proc. Natl. Acad. Sci. USA 77 (1980) 2819-2823] and respond to DNA damage by producing beta-galactosidase. They can be used to characterize restriction systems and screen for restriction endonuclease mutants. Applications include the study of other enzymes involved in DNA metabolism, such as DNA methyltransferases, topoisomerases, recombinases, and DNA replication and repair enzymes.

Full Text

Duke Authors

Cited Authors

  • Heitman, J; Model, P

Published Date

  • July 15, 1991

Published In

Volume / Issue

  • 103 / 1

Start / End Page

  • 1 - 9

PubMed ID

  • 1908806

International Standard Serial Number (ISSN)

  • 0378-1119

Digital Object Identifier (DOI)

  • 10.1016/0378-1119(91)90383-m


  • eng

Conference Location

  • Netherlands