Immunophilins interact with calcineurin in the absence of exogenous immunosuppressive ligands.

Journal Article (Journal Article)

The peptidyl-prolyl isomerases FKBP12 and cyclophilin A (immunophilins) form complexes with the immunosuppressants FK506 and cyclosporin A that inhibit the phosphatase calcineurin. With the yeast two hybrid system, we detect complexes between FKBP12 and the calcineurin A catalytic subunit in both the presence and absence of FK506. Mutations in FKBP12 surface residues or the absence of the calcineurin B regulatory subunit perturb the FK506-dependent, but not the ligand-independent, FKBP12-calcineurin complex. By affinity chromatography, both FKBP12 and cyclophilin A bind calcineurin A in the absence of ligand, and FK506 and cyclosporin A respectively potentiate these interactions. Both in vivo and in vitro, the peptidyl-prolyl isomerase active sites are dispensable for ligand-independent immunophilin-calcineurin complexes. Lastly, by genetic analyses we demonstrate that FKBP12 modulates calcineurin functions in vivo. These findings reveal that immunophilins interact with calcineurin in the absence of exogenous ligands and suggest that immunosuppressants may take advantage of the inherent ability of immunophilins to interact with calcineurin.

Full Text

Duke Authors

Cited Authors

  • Cardenas, ME; Hemenway, C; Muir, RS; Ye, R; Fiorentino, D; Heitman, J

Published Date

  • December 15, 1994

Published In

Volume / Issue

  • 13 / 24

Start / End Page

  • 5944 - 5957

PubMed ID

  • 7529175

Pubmed Central ID

  • PMC395570

International Standard Serial Number (ISSN)

  • 0261-4189

Digital Object Identifier (DOI)

  • 10.1002/j.1460-2075.1994.tb06940.x


  • eng

Conference Location

  • England